Lonomia obliqua (Lepidoptera, Saturniidae) caterpillar bristle extract induces direct lysis by cleaving erythrocyte membrane glycoproteins

Autor(es)

Seibert, Carla Simone ^[1] ; Santoro, Marcelo L. ^[1] ; Tambourgi, Denise Vilarinho ^[2] ; Sampaio, Sandra Coccuzzo ^[1] ; Takahashi, Hilton Kenji ^[3] ; Peres, Carmem Maldonado ^[3] ; Curi, Rui ^[3] ; Sano-Martins, Ida S. ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Fisiopatol, BR-05503900 Sao Paulo - Brazil

^[2] Inst Butantan, Lab Imunoquim, BR-05503900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Lab Fisiol & Biofis, BR-09500900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 55, n. 7, p. 1323-1330, JUN 15 2010.
• Citações Web of Science: 5

Resumo

Lonomia obliqua caterpillar bristle extract induces hemolysis in vitro on washed human and rat erythrocytes, in either the absence or presence of exogenous lecithin. In the former condition, phospholipases A(2) are key enzymes involved in hemolysis. However, the mechanism whereby this extract causes direct hemolysis is not known. Thus, the aim of this study was to investigate the hemolytic mechanism of the crude extract of the caterpillar L obliqua on human erythrocytes in the absence of lecithin. The extract significantly increased the erythrocyte osmotic fragility and promoted the removal of glycophorins A and C, and band 3 from the erythrocyte membrane. The use of Ca(2+) and Mg(2+) ions significantly potentiated glycoprotein removal, remarkably of erythrocyte band 3. The composition of fatty acids was analyzed by HPLC in both L obliqua caterpillar bristle extract and human erythrocyte membranes incubated with the extract. The levels of unsaturated fatty acids were remarkably augmented in erythrocytes incubated with the extract than in control erythrocytes, modifying thereby the saturated/unsaturated fatty acid ratio. Altogether, evidence is provided here that the interplay of at least three mechanisms of action accounts for the direct activity of the bristle extract on erythrocyte membrane, leading to hemolysis: the removal of glycoproteins and band 3; the insertion of fatty acids; and the action of phospholipases. Such mechanisms might affect erythrocyte flexibility and deformability, which may induce hemolysis by increasing erythrocyte fragility. However, whether the direct hemolytic activity of L obliqua caterpillar is the major cause of intravascular hemolysis during envenomation still needs further investigation. (C) 2010 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/02996-8 - Venenos de serpentes Bothrops jararaca recém-nascidas e adultas: comparação entre suas atividades enzimáticas e biológicas, imunoneutralização, composição química e capacidade de induzir alterações hemostáticas
• Beneficiário: Marcelo Larami Santoro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 01/07643-7 - Identificação e caracterização das toxinas com atividade hemolítica da lagarta Lonomia obliqua e da glândula produtora das toxinas: estudo bioquímico e morfológico
• Beneficiário: Ida Sigueko Sano Martins
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 00/11432-9 - Mecanismo(s) de ação hemolítica do extrato das cerdas da lagarta (Lonomia obliqua): identificação e caracterização funcional e bioquímica da(s) fração(ões)
• Beneficiário: Carla Simone Seibert
• Modalidade de apoio: Bolsas no Brasil - Doutorado