Inhibitory effect of silibinin on tumour necrosis factor-alpha and hydrogen peroxide production by human monocytes

Autor(es)

Bannwart, C. F. ^[1] ; Peracoli, J. C. ^[2] ; Nakaira-Takahagi, E. ^[1] ; Peracoli, M. T. S. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Dept Microbiol & Immunol, Biosci Inst, UNESP, BR-18618000 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, Dept Gynecol & Obstet, Botucatu Med Sch, UNESP, BR-18618000 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NATURAL PRODUCT RESEARCH; v. 24, n. 18, p. 1747-1757, 2010.
• Citações Web of Science: 13

Resumo

Silibinin is a chemically defined flavonoid and the main active component of silymarin, a polyphenolic complex from Silybum marianum, which has anti-inflammatory, hepatoprotective and anticarcinogenic properties. Monocytes obtained from healthy individuals were incubated with silibinin to evaluate cell viability, hydrogen peroxide (H2O2) release and tumour necrosis factor-alpha (TNF-) production by these cells. The duration of treatment and different silibinin concentrations had no significant effect on cell viability. Monocytes showed a dose-dependent inhibitory effect on H2O2 release by phorbol myristate acetate-stimulated monocytes in silibinin concentrations ranging from 6.25 to 50 mu g mL-1. Significant inhibition of TNF- production by lipopolysaccharide-stimulated monocytes was observed at concentrations of 12.5, 50 and 100 mu g mL-1 of silibinin. These results suggest that silibinin exerts antioxidant and anti-inflammatory properties on human monocytes through an inhibitory effect on H2O2 release and on TNF- production, respectively. (AU)

• Processo FAPESP: 03/10476-0 - Efeito da silimarina (silybum marianum) sobre a producao de fator de necrose tumoral alfa e peroxido de hidrogenio por monocitos de sangue periferico de individuos saudaveis.
• Beneficiário: Camila Ferreira Bannwart Castro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica