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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Inhibitory effect of silibinin on tumour necrosis factor-alpha and hydrogen peroxide production by human monocytes

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Author(s):
Bannwart, C. F. [1] ; Peracoli, J. C. [2] ; Nakaira-Takahagi, E. [1] ; Peracoli, M. T. S. [1]
Total Authors: 4
Affiliation:
[1] Sao Paulo State Univ, Dept Microbiol & Immunol, Biosci Inst, UNESP, BR-18618000 Botucatu, SP - Brazil
[2] Sao Paulo State Univ, Dept Gynecol & Obstet, Botucatu Med Sch, UNESP, BR-18618000 Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: NATURAL PRODUCT RESEARCH; v. 24, n. 18, p. 1747-1757, 2010.
Web of Science Citations: 13
Abstract

Silibinin is a chemically defined flavonoid and the main active component of silymarin, a polyphenolic complex from Silybum marianum, which has anti-inflammatory, hepatoprotective and anticarcinogenic properties. Monocytes obtained from healthy individuals were incubated with silibinin to evaluate cell viability, hydrogen peroxide (H2O2) release and tumour necrosis factor-alpha (TNF-) production by these cells. The duration of treatment and different silibinin concentrations had no significant effect on cell viability. Monocytes showed a dose-dependent inhibitory effect on H2O2 release by phorbol myristate acetate-stimulated monocytes in silibinin concentrations ranging from 6.25 to 50 mu g mL-1. Significant inhibition of TNF- production by lipopolysaccharide-stimulated monocytes was observed at concentrations of 12.5, 50 and 100 mu g mL-1 of silibinin. These results suggest that silibinin exerts antioxidant and anti-inflammatory properties on human monocytes through an inhibitory effect on H2O2 release and on TNF- production, respectively. (AU)