Effects of supernatant from monocytes of pre-eclamptic women treated with sibilin on oxidative stress and nitric oxide availability in endothelial cells

• Grant number: 19/10250-0
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: June 14, 2019
• End date: September 08, 2019
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Maria Terezinha Serrão Peraçoli
• Grantee: Virgínia Juliani Gomes
• Supervisor: Mattias Carlstrom
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: Karolinska Institutet, Sweden
• Associated to the scholarship: 17/26053-4 - Monosodium urate and silibinin modulation of monocytes from preeclamptic women and THP-1 lineage of human monocytes, BP.MS

Abstract

Preeclampsia (PE) is a gestational specific syndrome associated with endothelial celldysfunction, elevated serum levels of inflammatory cytokines and oxidative stress. Previousresults from our research group showed that PE is marked by oxidative stress and the monocytesfrom maternal peripheral blood might be an important source of free radicals and inflammatorycytokines in this disease. Nitric oxide (NO) is a biological mediator able to induce vasodilation.The imbalance between NO and reactive oxygen species (ROS) seems to play an important rolein the pathogenesis of PE and may be improved by the administration of silibinin, a flavonoidwith antioxidant properties. Therefore, this project aims to evaluate whether the supernatantof monocytes from normotensive and preeclamptic pregnant women treated or not withsilibinin is able to modulate the generation of oxidative stress and improve the nitric oxideavailability in human umbilical vein endothelial cells (HUVEC). HUVEC will be cultured untilreaching 80% confluence and then incubate for 24 hours in medium with 20% (v/v) ofsupernatant of monocytes from normotensive and preeclamptic pregnant women cultured withor without silibinin. The cells and supernatant generated will be used to perform the assays.Electron paramagnetic resonance (EPR) and high performance liquid chromatography (HPLC)system will be used to assess NO and its oxidized metabolites nitrate and nitrite. Determinationof TBARS (Thiobarbituric acid reactive substances) will be employed to evaluate ROS-mediatedlipidic peroxidation. Hemeoxigenase-1 (HO-1) concentrations in cell supernatants will bemeasured using the enzyme-linked immunosorbent assay kit. Statistical analysis (ANOVA or ttest) will be performed employing parametric or nonparametric tests, with p value of <0.05considered significant.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES, VIRGINIA JULIANI; NUNES, PRISCILA REZECK; HAWORTH, SARAH MCCANN; SANDRIM, VALERIA CRISTINA; PERACOLI, JOSE CARLOS; PERACOLI, MARIA TEREZINHA S.; CARLSTROM, MATTIAS. Monocytes from preeclamptic women previously treated with silibinin attenuate oxidative stress in human endothelial cells. HYPERTENSION IN PREGNANCY, v. 40, n. 2, FEB 2021. (19/10250-0)