Short-chain fatty acids stimulate the migration of neutrophils to inflammatory sites

Autor(es)

Vinolo, Marco A. R. ^[1] ; Rodrigues, Hosana G. ^[1] ; Hatanaka, Elaine ^[1] ; Hebeda, Cristina B. ^[2] ; Farsky, Sandra H. P. ^[2] ; Curi, Rui ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Clin & Toxicol Anal, Fac Pharmaceut Sci, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Clinical Science; v. 117, n. 9-10, p. 331-338, NOV 2009.
• Citações Web of Science: 62

Resumo

SCFAs (short-chain fatty acids) are produced by anaerobic bacterial fermentation. Increased concentrations of these fatty acids are observed in inflammatory conditions, such as periodontal disease, and at sites of anaerobic infection. In the present study, the effect of the SCFAs acetate, propionate and butyrate on neutrophil chemotaxis and migration was investigated. Experiments were carried out in rats and in vitro. The following parameters were measured: rolling, adherence, expression of adhesion molecules in neutrophils (L-selectin and beta 2 integrin), transmigration, air pouch influx of neutrophils and production of cytokines {[}CINC-2 alpha beta (cytokine-induced neutrophil chemoattractant-2 alpha beta), IL-1 beta (interleukin-1 beta), MIP-1 alpha (macrophage inflammatory protein-1 alpha) and TNF-alpha (tumour necrosis factor-alpha)]. SCFAs induced in vivo neutrophil migration and increased the release of CINC-2 alpha beta into the air pouch. These fatty acids increased the number of rolling and adhered cells as evaluated by intravital microscopy. SCFA treatment increased L-selectin expression on the neutrophil surface and L-selectin mRNA levels, but had no effect on the expression of beta 2 integrin. Propionate and butyrate also increased in vitro transmigration of neutrophils. These results indicate that SCFAs produced by anaerobic bacteria raise neutrophil migration through increased L-selectin expression on neutrophils and CINC-2 alpha beta release. (AU)

• Processo FAPESP: 04/12137-1 - Estudo dos mecanismos de ação dos ácidos graxos em leucócitos
• Beneficiário: Rui Curi
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 06/00372-1 - Papel do GPR43 na função de macrófagos: possível mecanismo de ação dos ácidos graxos de cadeia curta
• Beneficiário: Marco Aurélio Ramirez Vinolo
• Linha de fomento: Bolsas no Brasil - Doutorado Direto