Signaling pathways associated with the expression of inflammatory mediators activated during the course of two models of experimental periodontitis

Autor(es)

de Aquino, Sabrina Garcia ^[1] ; Manzolli Leite, Fabio Renato ^[1] ; Stach-Machado, Dagmar Ruth ^[2] ; Francisco da Silva, Juliete Aparecida ^[2] ; Spolidorio, Luis Carlos ^[3] ; Rossa, Jr., Carlos ^[1]

Afiliação do(s) autor(es)

^[1] UNESP, Dept Diag & Surg, Sch Dent, BR-14801903 Araraquara, SP - Brazil

^[2] Univ Estadual Campinas, Dept Microbiol & Immunol, Inst Biol, BR-13083862 Campinas, SP - Brazil

^[3] UNESP, Dept Physiol & Pathol, Sch Dent, BR-14801903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 84, n. 21-22, p. 745-754, MAY 22 2009.
• Citações Web of Science: 42

Resumo

Aims: Evaluate the signaling pathways associated with inflammatory mediators activated in two models of experimental periodontitis. Main methods: Two models were used: lipopolysaccharide (LPS) injections and ligature placement. Wistar rats were used and 30 mu g LIPS from Escherichia coli was injected twice a week into the palatal aspect of the upper molars. Ligatures were placed around lower first molars. A control group received injections of PBS on the palatal gingivae whereas no ligatures were placed on the lower molars. Samples were collected 5,15 and 30 days and processed for analysis by Western blotting and stereometry. Key findings: The ligature model was associated with rapid and transient activation of extracellular-regulated kinases (ERK) and p38 mitogen-activated protein kinase (MAPK) as well as of nuclear factor kappa B (NF-kappa B). Activation of these signaling pathways on the LPS model was delayed but sustained throughout the 30-day experimental period. Inflammatory changes induced by both models were similar; however there was a significant reduction on inflammation degree on the ligature model, which paralleled the decrease observed on the activation of the signaling pathways. Activation of signal transducer and activator of transcription (SEAT)-3 by phosphorylation of Tyrosine residues and of SPAT-5 was observed only on the ligature model. Significance: Regulation of gene expression results from the activation of signaling pathways initiated by receptor-ligand binding of external antigens and also of cytokines produced by the host immune system. Understanding the signaling pathways relevant fora given condition may provide information useful for novel therapeutic approaches. (C) 2009 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 05/04428-9 - Efeito da inibição da IL-2 com FK-506 na expressão gênica de RANKL e OPG na doença periodontal induzida por ligadura: estudo in vivo e in vitro
• Beneficiário: Carlos Rossa Junior
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/07283-4 - Efeito da inibição de IL-2 com FK-506 na expressão gênica de RANKL e OPG na doença periodontal induzida por ligadura: estudo in vivo e in vitro
• Beneficiário: Angélica Martins
• Linha de fomento: Bolsas no Brasil - Iniciação Científica