Characterization of a 21 kDa protein from Trypanosoma cruzi associated with mammalian cell invasion

Autor(es):Mostrar menos -

da Silva, Claudio V. ^[1] ; Kawashita, Silvia Y. ^[1] ; Probst, Christian M. ^{[2, 3]} ; Dallagiovanna, Bruno ^{[2, 3]} ; Cruz, Mario C. ^[1] ; da Silva, Erika A. ^[1] ; Souto-Padron, Thais C. B. S. ^[4] ; Krieger, Marco A. ^{[2, 3]} ; Goldenberg, Samuel ^{[2, 3]} ; Briones, Marcelo R. S. ^[1] ; Andrews, Norma W. ^[5] ; Mortara, Renato A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo - Brazil

^[2] Fiocruz MS, Inst Biol Mol Parana, Curitiba, Parana - Brazil

^[3] Fiocruz MS, ICC, Curitiba, Parana - Brazil

^[4] Univ Fed Rio de Janeiro, Dept Microbiol, BR-21941 Rio De Janeiro - Brazil

^[5] Yale Univ, Sch Med, Sect Microbial Pathogenesis, New Haven, CT - USA

• Tipo de documento: Artigo Científico
• Fonte: Microbes and Infection; v. 11, n. 5, p. 563-570, APR 2009.
• Citações Web of Science: 14

Resumo

Trypanosoma cruzi genomic database was screened for hypothetical proteins that showed high probability of being secreted or membrane anchored and thus, likely involved in host-cell invasion. A sequence that codes for a 21 kDa protein that showed high probability of being secreted was selected. After cloning this protein sequence, the results showed that it was a ubiquitous protein and secreted by extracellular amastigotes. The recombinant form (P21-His(6)) adhered to HeLa cells in a dose-dependent manner. Pretreatment of host cells with P21-His(6) inhibited cell invasion by extracellular amastigotes from G and CL strains. On the other hand, when the protein was added to host cells at the same time as amastigotes, an increase in cell invasion was observed. Host-cell pretreatment with P21-His(6) augmented invasion by metacyclic trypomastigotes. Moreover, polyclonal antibody anti-P21 inhibited invasion only by extracellular amastigotes and metacyclic trypomastigotes from G strain. These results suggested that P21 might be involved in T. cruzi cell invasion. We hypothesize that P21 could be secreted in the juxtaposition parasite-host cell and triggers signaling events yet unknown that lead to parasite internalization. (C) 2009 Elsevier Masson SAS. All rights reserved. (AU)

• Processo FAPESP: 03/12962-0 - Estudos da invasão de células em cultura por formas amastigotas extracelulares de cepas de Trypanosoma Cruzi isoladas de pacientes chagásicos
• Beneficiário: Claudio Vieira da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado