| Full text | |
| Author(s): Show less - |
da Silva, Claudio V.
[1]
;
Kawashita, Silvia Y.
[1]
;
Probst, Christian M.
[2, 3]
;
Dallagiovanna, Bruno
[2, 3]
;
Cruz, Mario C.
[1]
;
da Silva, Erika A.
[1]
;
Souto-Padron, Thais C. B. S.
[4]
;
Krieger, Marco A.
[2, 3]
;
Goldenberg, Samuel
[2, 3]
;
Briones, Marcelo R. S.
[1]
;
Andrews, Norma W.
[5]
;
Mortara, Renato A.
[1]
Total Authors: 12
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo - Brazil
[2] Fiocruz MS, Inst Biol Mol Parana, Curitiba, Parana - Brazil
[3] Fiocruz MS, ICC, Curitiba, Parana - Brazil
[4] Univ Fed Rio de Janeiro, Dept Microbiol, BR-21941 Rio De Janeiro - Brazil
[5] Yale Univ, Sch Med, Sect Microbial Pathogenesis, New Haven, CT - USA
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Microbes and Infection; v. 11, n. 5, p. 563-570, APR 2009. |
| Web of Science Citations: | 14 |
| Abstract | |
Trypanosoma cruzi genomic database was screened for hypothetical proteins that showed high probability of being secreted or membrane anchored and thus, likely involved in host-cell invasion. A sequence that codes for a 21 kDa protein that showed high probability of being secreted was selected. After cloning this protein sequence, the results showed that it was a ubiquitous protein and secreted by extracellular amastigotes. The recombinant form (P21-His(6)) adhered to HeLa cells in a dose-dependent manner. Pretreatment of host cells with P21-His(6) inhibited cell invasion by extracellular amastigotes from G and CL strains. On the other hand, when the protein was added to host cells at the same time as amastigotes, an increase in cell invasion was observed. Host-cell pretreatment with P21-His(6) augmented invasion by metacyclic trypomastigotes. Moreover, polyclonal antibody anti-P21 inhibited invasion only by extracellular amastigotes and metacyclic trypomastigotes from G strain. These results suggested that P21 might be involved in T. cruzi cell invasion. We hypothesize that P21 could be secreted in the juxtaposition parasite-host cell and triggers signaling events yet unknown that lead to parasite internalization. (C) 2009 Elsevier Masson SAS. All rights reserved. (AU) | |