Influence of dissolved oxygen and shear conditions on clavulanic acid production by Streptomyces clavuligerus

Autor(es)

Rosa, J. C. ^[1] ; Baptista Neto, A. ; Hokka, C. O. ; Badino, A. C. ^[4]

Afiliação do(s) autor(es)

^[1] Universidade Federal de São Carlos (UFSCAR). Centro de Ciências Exatas e de Tecnologia - Brasil

^[4] Universidade Federal de São Carlos (UFSCAR). Centro de Ciências Exatas e de Tecnologia - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Bioprocess and Biosystems Engineering; v. 27, n. 2, p. 99-104, Apr. 2005.
• Área do conhecimento: Engenharias - Engenharia Química
• Assunto(s): Bactérias Streptomyces clavuligerus Ácido clavulânico

Resumo

Clavulanic acid (CA), a potent beta-lactamase inhibitor, is produced by a filamentous bacterium. Here, the effect of DO and shear, expressed as impeller tip velocity, on CA production was examined. Cultivations were performed in a 4 L fermentor with speeds of 600, 800 and 1,000 rpm and a fixed air flow rate (0.5 vvm). Also, cultivation with automatic control of dissolved oxygen, at 50% air saturation, by varying stirrer speed and using a mixture of air and O2 (10% v/v) in the inlet gas, and a cultivation with fixed stirrer speed of 800 rpm and air flow rate of 0.5 vvm, enriched with 10% v/v O2, were performed. Significant variations in CA titer, CA production rate and O2 uptake-rate were observed. It was also found that the DO level has no remarkable effect on CA production once a critical level is surpassed. The most significant improvement in CA production was related to high stirrer speeds. (AU)

• Processo FAPESP: 00/01383-0 - Estudo da influência da concentração de oxigênio dissolvido na produção de ácido clavulânico por Streptomyces clavuligerus
• Beneficiário: João Carlos Rosa
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 03/11722-5 - Producao de acido clavulanico em biorreatores convencional e airlift.
• Beneficiário: Alberto Colli Badino Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular