| Full text | |
| Author(s): |
Total Authors: 4
|
| Affiliation: | [1] Universidade Federal de São Carlos (UFSCAR). Centro de Ciências Exatas e de Tecnologia - Brasil
[4] Universidade Federal de São Carlos (UFSCAR). Centro de Ciências Exatas e de Tecnologia - Brasil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Bioprocess and Biosystems Engineering; v. 27, n. 2, p. 99-104, Apr. 2005. |
| Field of knowledge: | Engineering - Chemical Engineering |
| Abstract | |
Clavulanic acid (CA), a potent beta-lactamase inhibitor, is produced by a filamentous bacterium. Here, the effect of DO and shear, expressed as impeller tip velocity, on CA production was examined. Cultivations were performed in a 4 L fermentor with speeds of 600, 800 and 1,000 rpm and a fixed air flow rate (0.5 vvm). Also, cultivation with automatic control of dissolved oxygen, at 50% air saturation, by varying stirrer speed and using a mixture of air and O2 (10% v/v) in the inlet gas, and a cultivation with fixed stirrer speed of 800 rpm and air flow rate of 0.5 vvm, enriched with 10% v/v O2, were performed. Significant variations in CA titer, CA production rate and O2 uptake-rate were observed. It was also found that the DO level has no remarkable effect on CA production once a critical level is surpassed. The most significant improvement in CA production was related to high stirrer speeds. (AU) | |