Endurance training induces depot-specific changes in IL-10/TNF-alpha ratio in rat adipose tissue

Autor(es)

Lira, F. S. ^[1] ; Rosa, J. C. ^[2] ; Yamashita, A. S. ^[1] ; Koyama, C. H. ^[1] ; Batista, Jr., M. L. ^{[3, 1]} ; Seelaender, M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Mol Biol Cell Grp, BR-05508900 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Div Nutr Physiol, Dept Physiol, Sao Paulo - Brazil

^[3] Univ Mogi das Cruzes, Sch Phys Educ, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CYTOKINE; v. 45, n. 2, p. 80-85, FEB 2009.
• Citações Web of Science: 64

Resumo

White adipose tissue (WAT) is the source of pro- and anti-inflammatory cytokines and recently, it has been recognized as an important source of interleukin 10 (IL-10). Acute physical exercise is known to induce an anti-inflammatory cytokine profile, however, the effect of chronic physical exercise on the production of IL-10 by WAT has never been examined. We assessed IL-10 and TNF-alpha concentration in WAT of rats engaged in endurance training. Animals were randomly assigned to either a sedentary control group (S, n = 7) or an endurance trained group (T, n = 8). Trained rats ran on a treadmill 5 days/wk for 8 wk (55-65% VO(2max). Detection of IL-10 and TNF-alpha protein and mRNA expression, as well as the gene expression of PPAR-gamma, and immunocytochemistry to detect mononuclear phagocytes were carried out. A reduction in absolute retroperitoneal adipose tissue (RPAT) weight in T (44%; p < 0.01), when compared with S was observed. IL-10 concentration was increased (1.5-fold, p < 0.05), to a higher extent than that of TNF-alpha (66%. p < 0.05) in the mesenteric adipose tissue (MEAT) of the trained group, while no change related to training was observed in RPAT. In MEAT, IL-10/TNF-alpha ratio was increased in T, when compared with S (30%; p < 0.05). PPAR-gamma gene expression was increased in T (1.1-fold; p < 0.01), when compared with S in the same adipose depot. No monocyte infiltration was found. In conclusion, exercise training induced increased IL-10 expression in the mesenteric depot, resulting in a modified IL-10/TNF-alpha ratio. We also conclude that WAT presents a depot-specific response to endurance training regarding the studied aspects. (C) 2008 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 05/01445-0 - Papel dos PPARs na caquexia associada ao câncer
• Beneficiário: Marilia Cerqueira Leite Seelaender
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 04/11588-0 - Expressao de receptores de prostanoides em hepatocitos perioportais e perivenosos: efeitos de mediadores da caquexia associada ao cancer.
• Beneficiário: Marilia Cerqueira Leite Seelaender
• Modalidade de apoio: Auxílio à Pesquisa - Regular