Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples

Autor(es)

Marinheiro, Juliana Cristina ^[1] ; Sanalios, Roberta Braga ^[1] ; dos Santos, Daniela Carvalho ^[1] ; da Costa, Cristovao Alves ^[2] ; Harsi, Charlotte Marianna ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Lab Biol Mol Adenovirus, BR-05508900 Sao Paulo - Brazil

^[2] Inst Nacl de Pesquisas da Amazonia, Lab Virol Trop, Manaus, Amazonas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 104, n. 1, p. 118-120, FEB 2009.
• Citações Web of Science: 1

Resumo

Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time. (AU)

• Processo FAPESP: 06/56482-0 - Variabilidade genética de adenovírus humanos das espécies B e C, isolados de caso de infecção respiratória aguda, em São Paulo, de 1995 a 2005
• Beneficiário: Charlotte Marianna Hársi
• Modalidade de apoio: Auxílio à Pesquisa - Regular