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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples

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Author(s):
Marinheiro, Juliana Cristina [1] ; Sanalios, Roberta Braga [1] ; dos Santos, Daniela Carvalho [1] ; da Costa, Cristovao Alves [2] ; Harsi, Charlotte Marianna [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Lab Biol Mol Adenovirus, BR-05508900 Sao Paulo - Brazil
[2] Inst Nacl de Pesquisas da Amazonia, Lab Virol Trop, Manaus, Amazonas - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 104, n. 1, p. 118-120, FEB 2009.
Web of Science Citations: 1
Abstract

Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time. (AU)