Analysis of the gene expression patterns in peripherical mononuclear cells of infants with respiratory syncytial virus infection

• Grant number: 12/22854-9
• Support Opportunities: Regular Research Grants
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Sandra Elisabete Vieira
• Grantee: Sandra Elisabete Vieira
• Host Institution: Hospital Universitário (HU). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Alfredo Elias Gilio ; Carlos Alberto Moreira Filho ; Edison Luiz Durigon ; Marina Baquerizo Martinez ; Silvia Yumi Bando Takahara

Abstract

The study´s main goal is to identify the pattern of gene expression and transcriptional interaction and interactome networks in respiratory syncytial virus (RSV) infection, by analyzing the transcriptome of peripheral blood mononuclear cells of RSV infected infants compared with parainfluenza virus infection and with a control group of uninfected infants. A cohort study will select children aged between 0 and 6 months, admitted to the University Hospital with acute infection of the respiratory system. Preterm infants, children with a family history of asthma / atopy, patients with comorbidities and / or corticosteroid or immunosuppressant use in the last 30 days will be excluded. Among the selected patients 12 with RSV and 12 with parainfluenza virus detected by viral test in nasopharyngeal secretions (immunofluorescence) will be included in the study. In addition to immunofluorescence (for RSV, influenza, parainfluenza virus and adenovirus), also PCR / RT-PCR (for RSV, metapneumovirus, parainfluenza virus, influenza, rhinovirus and adenovirus) and RSV sequencing/parainfluenza typing will be performed for the included patients. Blood samples will be collected of infants for blood count, blood culture and mononuclear cells RNA extraction for analysis of global gene expression and construction of transcriptional interaction networks. The selection of 12 healthy children for the control group will take place among routine visits including those underwent routine blood sampling for reasons others than infectious or inflammatory events. Clinical protocols will be filled up during the hospitalization period. Follow up phone contacts will be performed one week after the date of discharge from hospital or one week after the date of inclusion of non-hospitalized infants. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIEIRA, SANDRA E.; BANDO, SILVIA Y.; DE PAULIS, MILENA; OLIVEIRA, DANIELLE B. L.; THOMAZELLI, LUCIANO M.; DURIGON, EDISON L.; MARTINEZ, MARINA B.; MOREIRA-FILHO, CARLOS ALBERTO. Distinct transcriptional modules in the peripheral blood mononuclear cells response to human respiratory syncytial virus or to human rhinovirus in hospitalized infants with bronchiolitis. PLoS One, v. 14, n. 3, MAR 7 2019. (15/22308-2, 12/22854-9)

DE PAULIS, MILENA; LEAL OLIVEIRA, DANIELLE BRUNA; THOMAZELLI, LUCIANO MATSUMIYA; FERRARO, ALEXANDRE ARCHANJO; DURIGON, EDISON LUIZ; VIEIRA, SANDRA E.. The importance of viral load in the severity of acute bronchiolitis in infants. Clinics, v. 76, 2021. (12/22854-9)