Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Bothrops jararaca fibrinogen and its resistance to hydrolysis evoked by snake venoms

Texto completo
Autor(es):
Vieira, Carolina O. [1, 2] ; Tanaka, Aparecida S. [3] ; Sano-Martins, Ida S. [1, 2] ; Morais, Karen B. [1, 2] ; Santoro, Marcelo L. [1] ; Tanaka-Azevedo, Anita M. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Inst Butantan, Lab Fisiopatol, BR-05503900 Sao Paulo - Brazil
[2] IBUSP, Dept Physiol, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Biochem, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY; v. 151, n. 4, p. 428-432, DEC 2008.
Citações Web of Science: 3
Resumo

Fibrinogen is an essential protein involved in several steps of hemostasis, being associated with the final steps of the blood coagulation mechanism. Herein, we describe the purification and characterization of a reptile fibrinogen, obtained from Bothrops jararaca plasma. Native B. jararaca fibrinogen showed a molecular mass of 372 kDa, and the reduced and alkylated fibrinogen molecule showed three chains of 71, 60 and 55 kDa, which are similar to the molecular masses of human and bovine Act, BP and gamma fibrinogen chains. Remarkably, B. jararaca fibrinogen was clotted by bovine thrombin, but B. jararaca, Crotalus durissus terrificus and Lachesis muta rhombeata venoms could not induce its clotting or hydrolysis. Thus, despite the similarities between B. jararaca and mammalian fibrinogens, the former shows distinctive features, which protect B. jararaca snakes from accidental envenomation. (c) 2008 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 04/02224-4 - Estudo de estrutura e função de proteínas envolvidas no mecanismo de coagulação da serpente Bothrops jararaca
Beneficiário:Anita Mitico Tanaka-Azevedo
Modalidade de apoio: Auxílio à Pesquisa - Regular