Effects of alternagin-C from Bothrops alternatus on gene expression and activity of metalloproteinases in regenerating skeletal muscle

Autor(es)

Quagliotti Durigan, Joao Luiz ^[1] ; Peviani, Sabrina Messa ^[1] ; Russo, Thiago Luiz ^[1] ; Delfino, Gabriel Borges ^[1] ; Ribeiro, Juliana Uema ^[2] ; Cominetti, Marcia Regina ^[2] ; Selistre-de-Araujo, Heloisa Sobreiro ^[2] ; Salvini, Tania Fatima ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Phys Therapy, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Physiol Sci, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 52, n. 6, p. 687-694, NOV 2008.
• Citações Web of Science: 14

Resumo

This study evaluated the effects of alternagin-C (ALT-C) on mRNA levels of VEGF, MyoD and matrix metalloproteinase 2 (MMP-2) and on activity of MMPs in injured tibialis anterior (TA) muscle induced by cryolesioning in rats. Thirty-six Wistar rats (3 months old, 258.9 +/- 27 g) were divided into five groups: (1) control group; (2) injured TA and analyzed 3 days later; (3) injured TA treated with ALT-C and analyzed 3 days later; (4) injured TA and analyzed 7 days later and (5) injured TA treated with ALT-C and analyzed 7 days later. The injured muscle received 25 mu l of ALT-C (50 nM). The injured and uninjured muscle areas were quantified by light microscopy. The MMP activity was evaluated through zymography, and mRNA of MyoD, VEGF and MMP-2 was assessed by quantitative polymerase chain reaction. ALT-C neither reduced the muscle injury area nor altered the pattern of MyoD and VEGF expression in injured muscles. However, ALT-C reduces both MMP-2 mRNA and gelatinolytic activity in injured muscles. The study indicates that ALT-C, at the tested concentrations, did not improve muscle regeneration process in rats. The effect on MMP-2 mRNA and gelatinolytic activity suggests that ALT-C changes the overall balance of ECM protein turnover during muscle regeneration. (c) 2008 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 04/06986-6 - Papel biológico do domínio desintegrina de uma ADAM (a Desintegrin And Metalloproteinase) humana
• Beneficiário: Márcia Regina Cominetti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado