Soybean and sunflower oil-induced insulin resistance correlates with impaired GLUT4 protein expression and translocation specifically in white adipose tissue

Autor(es)

Poletto, Ana Claudia ^[1] ; Anhe, Gabriel Forato ^[2] ; Eichler, Paula ^[1] ; Takahashi, Hilton Kenji ^[1] ; Furuya, Daniela Tomie ^[1] ; Okamoto, Maristela Mitiko ^[1] ; Curi, Rui ^[1] ; Machado, Ubiratan Fabres ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Dept Pharmacol, Fac Med Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cell Biochemistry and Function; v. 28, n. 2, p. 114-121, MAR 2010.
• Citações Web of Science: 7

Resumo

Free fatty acids are known for playing a crucial role in the development of insulin resistance. High fat intake is known for impairing insulin sensitivity; however, the effect of vegetable-oil injections have never been investigated. The present study investigated the effects of daily subcutaneous injections (100 mu L) of soybean (SB) and sunflower (SF) oils, during 7 days. Both treated groups developed insulin resistance as assessed by insulin tolerance test. The mechanism underlying the SB- and SF-induced insulin resistance was shown to involve GLUT4. In SB- and SF-treated animals, the GLUT4 protein expression was reduced similar to 20% and 10 min after an acute it? vivo stimulus with insulin, the plasma membrane GLUT4 content was similar to 60% lower in white adipose tissue (WAT). No effects were observed in skeletal muscle. Additionally, both oil treatments increased mainly the content of palmitic acid (similar to 150%) in WAT, which can contribute to explain the GLUT4 regulations. Altogether, the present study collects evidence that those oil treatments might generate insulin resistance by targeting GLUT4 expression and translocation specifically in WAT. These alterations are likely to be caused due to the specific local increase in saturated fatty acids that occurred as a consequence of oil daily injections. Copyright (C) 2010 John Wiley \& Sons, Ltd. (AU)

• Processo FAPESP: 07/50554-1 - Transportadores de glicose e Diabetes mellitus: contribuição ao conhecimento da regulação da glicemia e do desenvolvimento de complicações
• Beneficiário: Ubiratan Fabres Machado
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 03/08548-3 - Efeito do óleo de soja e de girassol na sensibilidade insulínica e na expressão do gene do GLUT4
• Beneficiário: Ana Claudia Poletto
• Modalidade de apoio: Bolsas no Brasil - Mestrado