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(Referência obtida automaticamente do Google Scholar, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Detection of electronegative low density lipoprotein (LDL-) in plasma and atherosclerotic lesions by monoclonal antibody-based immunoassays

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Autor(es):
Damasceno‚ N.R.T. ; Sevanian‚ A. ; Apolinário‚ E. ; Oliveira‚ J. ; Fernandes‚ I. ; Abdalla‚ D.S.P.
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: CLINICAL BIOCHEMISTRY; v. 39, n. 1, p. 28-38, 2006.
Resumo

Objectives: To produce a monoclonal antibody (MAb) against electronegative LDL (LDL-) for detecting this modified lipoprotein in blood plasma and tissues. Design and methods: LDL was isolated from human blood plasma and used as an antigen for immunization of Balb/c mice. Lymphocytes of immunized mice were fused with myeloma cells (SP2/0) to obtain the hybridomas. LDL- was detected in blood plasma and atherosclerotic lesions of humans and rabbits by MAb-based ELISA and immunohistochemistry, respectively. Results: LDL- concentrations were higher (P < 0.05) in the blood plasma of hypercholesterolemic subjects (HC, 248 +/- 77 mg/dL of total cholesterol) than in normolipidemic subjects (NL, 173 +/- 82 mg/dL of total cholesterol) and rabbits (HC, 250 15 mg/dL of cholesterol versus NL, 81 +/- 12 mg/dL of cholesterol). Moreover, LDL- was detected in the atherosclerotic lesions of humans and rabbits. Conclusion: These MAb-based immunoassays are adequate to detect LDL- in biological samples and represent an important tool for investigating the role of LDL- in atherosclerosis. (c) 2005 The Canadian Society of Clinical Chemists. All rights reserved. (AU)

Processo FAPESP: 97/05090-3 - Possíveis conexões entre hipertensão e hipercolesterolemia, em relação à aterosclerose: vias de inativação do óxido nítrico e oxidação de lipoproteínas
Beneficiário:Dulcineia Saes Parra Abdalla
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 97/09686-8 - Influência das isoflavonas isoladas da soja na modificação oxidativa das lipoproteínas na aterosclerose experimental induzida pela caseína
Beneficiário:Nágila Raquel Teixeira Damasceno
Modalidade de apoio: Bolsas no Brasil - Doutorado