The Lectin ArtinM Induces Recruitment of Rat Mast Cells from the Bone Marrow to the Peritoneal Cavity

Autor(es)

de Almeida Buranello, Patricia Andressa ^[1] ; Isnard Moulin, Maria Raquel ^[1] ; Souza, Jr., Devandir Antonio ^[1] ; Jamur, Maria Celia ^[1] ; Roque-Barreira, Maria Cristina ^[1] ; Oliver, Constance ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Cell & Mol Biol & Pathogen Bioagents, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 5, n. 3, p. e9776, 2010.
• Citações Web of Science: 9

Resumo

Background: The D-mannose binding lectin ArtinM is known to recruit neutrophils, to degranulate mast cells and may have potential therapeutic applications. However, the effect of ArtinM on mast cell recruitment has not been investigated. Methodology: Male Wistar rats were injected i.p. with ArtinM or ConA (control). The ability of the lectin to degranulate peritoneal and mesenteric mast cells was examined. Recruitment of mast cells to the peritoneal cavity and mesentery after ArtinM injection was examined with or without depletion of peritoneal mast cells by distilled water. Results: ArtinM degranulated both peritoneal and mesentery mast cells in vitro. Three days after i.p. injection of the lectin there were reduced numbers of mast cells in the peritoneal lavage, while at 7 days post injection of ArtinM, the number of peritoneal mast cells was close to control values. Since immature mast cells are recruited from the bone marrow, the effect of the lectin on bone marrow mast cells was examined. Injection of ArtinM resulted in an increased number of mast cells in the bone marrow. To determine if degranulation of mast cells in the peritoneal cavity was required for the increase in bone marrow mast cells, the peritoneal cavity was depleted of mast cells with ultrapure water. Exposure to ArtinM increased the number of mast cells in the bone marrow of rats depleted of peritoneal mast cells. Conclusions: The ArtinM induced recruitment of mast cells from the bone marrow to the peritoneal cavity may partially explain the therapeutic actions of ArtinM. (AU)

• Processo FAPESP: 06/60642-2 - Efeitos biológicos e aplicações farmacêuticas de lectinas
• Beneficiário: Maria Cristina Roque Antunes Barreira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 05/01933-4 - O papel da lectina KM+ na proliferação, migração e maturação de mastócitos
• Beneficiário: Patrícia Andressa de Almeida Buranello
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 04/12545-2 - Estudo da dinâmica de migração de precursores de mastócitos e mastócitos imaturos da medula óssea para sítios periféricos
• Beneficiário: Devandir Antonio de Souza Junior
• Modalidade de apoio: Bolsas no Brasil - Mestrado