Characterization of Monocyte-Derived Dendritic Cells from Patients with Active and Treated Paracoccidioidomycosis

Autor(es)

Sato, P. K. ^[1] ; Oshiro, T. M. ^[2] ; Diogo, C. L. ^[1] ; Passos, E. C. ^[1] ; Shikanai-Yasuda, M. A. ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo HCFMUSP, Lab Med Invest Immunol LIM 48, Hosp Clin, Fac Med, BR-05403000 Sao Paulo - Brazil

^[2] HCFMUSP, Lab Med Invest Dermatol & Immunodeficiencies LIM, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med, Dept Infect & Parasit Dis, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Scandinavian Journal of Immunology; v. 74, n. 6, p. 609-618, DEC 2011.
• Citações Web of Science: 3

Resumo

Cellular immune responses are a significant defence mechanism in human paracoccidioidomycosis (PCM), an endemic mycosis in Latin America; however, little is known about the role of dendritic cells (DCs) in human PCM. We investigated monocyte-derived DCs from patients with treated (TP) and active PCM (AP) compared with healthy non-PCM donors (CO). DCs from the TP group showed higher expression of HLA-DR, CD86 and DC-SIGN compared with CO, whereas AP showed similar expression to CO. Production of IL-10 was downregulated by TNF-a in all groups and lower levels were observed in untreated DCs from AP compared with CO. Conversely, IL-12p40 was significantly upregulated in the DCs of the TP group. TNF-a-activated DCs from the CO group produced significantly lower levels of IL-12p40 when differentiated from magnetic-sorted monocytes (MACS) compared with adhered monocyte-derived DCs. This comparison in the TP group revealed similar levels of IL-12p40, suggesting a T cellindependent increase in the production of IL-12p40. Higher expression of surface molecules with increased IL-12p40 may indicate a better activation of DCs after the treatment of PCM. Our findings suggest that DCs may be crucial in the protective response to Paracoccidioides brasiliensis and that in vitro-generated DCs might be useful in enhancing antifungal immunity, especially during active PCM. (AU)

• Processo FAPESP: 04/14955-3 - Paracoccidioidomicose humana: reconhecimento de antígenos de Paracoccidioides brasiliensis por células dendríticas e linfócitos B de pacientes com diferentes formas clínicas: indução de resposta imune por anticorpo anti-idiotípico Ab2
• Beneficiário: Maria Aparecida Shikanai Yasuda
• Modalidade de apoio: Auxílio à Pesquisa - Regular