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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of Monocyte-Derived Dendritic Cells from Patients with Active and Treated Paracoccidioidomycosis

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Author(s):
Sato, P. K. [1] ; Oshiro, T. M. [2] ; Diogo, C. L. [1] ; Passos, E. C. [1] ; Shikanai-Yasuda, M. A. [1, 3]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo HCFMUSP, Lab Med Invest Immunol LIM 48, Hosp Clin, Fac Med, BR-05403000 Sao Paulo - Brazil
[2] HCFMUSP, Lab Med Invest Dermatol & Immunodeficiencies LIM, Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med, Dept Infect & Parasit Dis, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Scandinavian Journal of Immunology; v. 74, n. 6, p. 609-618, DEC 2011.
Web of Science Citations: 3
Abstract

Cellular immune responses are a significant defence mechanism in human paracoccidioidomycosis (PCM), an endemic mycosis in Latin America; however, little is known about the role of dendritic cells (DCs) in human PCM. We investigated monocyte-derived DCs from patients with treated (TP) and active PCM (AP) compared with healthy non-PCM donors (CO). DCs from the TP group showed higher expression of HLA-DR, CD86 and DC-SIGN compared with CO, whereas AP showed similar expression to CO. Production of IL-10 was downregulated by TNF-a in all groups and lower levels were observed in untreated DCs from AP compared with CO. Conversely, IL-12p40 was significantly upregulated in the DCs of the TP group. TNF-a-activated DCs from the CO group produced significantly lower levels of IL-12p40 when differentiated from magnetic-sorted monocytes (MACS) compared with adhered monocyte-derived DCs. This comparison in the TP group revealed similar levels of IL-12p40, suggesting a T cellindependent increase in the production of IL-12p40. Higher expression of surface molecules with increased IL-12p40 may indicate a better activation of DCs after the treatment of PCM. Our findings suggest that DCs may be crucial in the protective response to Paracoccidioides brasiliensis and that in vitro-generated DCs might be useful in enhancing antifungal immunity, especially during active PCM. (AU)