Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Evaluation of toxic effects of a diet containing fish contaminated with methylmercury in rats mimicking the exposure in the Amazon riverside population

Texto completo
Autor(es):
Grotto, Denise [1] ; Valentini, Juliana [1] ; Serpeloni, Juliana Mara [1] ; Ponte Monteiro, Patricia Alves [2] ; Latorraca, Elder Francisco [3] ; de Oliveira, Ricardo Santos [3] ; Greggi Antunes, Lusania Maria [1] ; Garcia, Solange Cristina [4] ; Barbosa, Jr., Fernando [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Patol, BR-14040903 Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Cirurgia & Anat, BR-14040903 Sao Paulo - Brazil
[4] Univ Fed Rio Grande do Sul, Dept Anal Clin, BR-90046900 Porto Alegre, RS - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Environmental Research; v. 111, n. 8, p. 1074-1082, NOV 2011.
Citações Web of Science: 17
Resumo

This study was designed to evaluate the effects of a diet rich in fish contaminated with MeHg, mimicking the typical diet of the Amazon riverside population, in rats. Animals were randomly assigned to one of three groups with eight rats in each group: Group I-control, received commercial ration: Group II-received a diet rich in uncontaminated fish; Group III-received a diet rich in fish contaminated with MeHg. Treatment time was 12 weeks. Oxidative stress markers were evaluated, as well as the effects of this diet on DNA stability, systolic blood pressure (SBP), nitric oxide (NO) levels and histological damage in different tissues. There was a significant increase in SBP values in rats fed with MeHg-contaminated fish diet after the 10th week of the treatment. As far as oxidative stress biomarkers are concerned, no differences were observed in reduced glutathione and protein carbonyl levels, glutathione peroxidase, catalase, superoxide dismutase or delta-aminolevulinate dehydratase activities between the groups of animals receiving contaminated and uncontaminated fish diets. On the other hand, malondialdehyde levels increased significantly in rats fed with contaminated fish. NO levels were similar in all groups. DNA migration showed augmented in rats exposed to contaminated fish and histopathological analyses showed weak but significant leukocyte infiltration. Thus, we conclude that the MeHg-contaminated fish diet induced a slight lipid peroxidation and genotoxicity. However, these effects seem to be much less pronounced than when rats are exposed to aqueous solution containing CH(3)HgCl. Our findings support the contention that the chemical form of MeHg in fish or fish nutrients such as polyunsaturated fatty acids, Se or vitamin E could minimize the toxic effects of MeHg exposure in fish-eating communities. (C) 2011 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 09/11102-3 - Especiação química de selênio e mercúrio em amostras biológicas para estudos de avaliação toxicológica e/ou nutricional humana
Beneficiário:Fernando Barbosa Júnior
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/07416-2 - Desenvolvimento de métodos para preparo de amostras e análise por especiação química de arsênio em amostras biológicas por HPLC-ICP-MS
Beneficiário:Fernando Barbosa Júnior
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 07/04538-4 - Identificação de genes diferencialmente expressos nas epilepsias farmaco-resistentes em crianças
Beneficiário:Helio Rubens Machado
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/07498-9 - Análise proteômica em cérebro e rins de ratos expostos a diferentes formas de mercúrio
Beneficiário:Fernando Barbosa Júnior
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 07/05221-4 - Comparação de parâmetros de estresse oxidativo entre ratos expostos a metilmercúrio em meio aquoso e a peixes contaminados com o metal
Beneficiário:Denise Grotto
Linha de fomento: Bolsas no Brasil - Doutorado