Food restriction and refeeding induces changes in lipid pathways and fat deposition in the adipose and hepatic tissues in rats with diet-induced obesity

Autor(es)

Duarte, Fernanda Oliveira ^{[1, 2]} ; Sene-Fiorese, Marcela ^[1] ; Cheik, Nadia Carla ^[3] ; Lopes Santa Maria, Adriana Simone ^[1] ; de Aquino, Jr., Antonio Eduardo ^[4] ; Oishi, Jorge Camargo ^[1] ; Rossi, Elizeu Antonio ^[5] ; de Oliveira Duarte, Ana Claudia Garcia ^{[1, 2]} ; Damaso, Ana Raimunda ^{[6, 7]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Educ Fis & Motricidade Humana, BR-13560 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Ctr Ciencias Biol & Saude, Dept Ciencias Fisiol, BR-13560 Sao Carlos, SP - Brazil

^[3] Univ Fed Uberlandia, Fac Educ Fis & Fisioterapia, BR-38400 Uberlandia, MG - Brazil

^[4] Univ Fed Sao Carlos, Programa Posgrad Biotecnol, BR-13560 Sao Carlos, SP - Brazil

^[5] Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Alimentos & Nutr, Araraquara, SP - Brazil

^[6] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biociencias, Sao Paulo - Brazil

^[7] Univ Fed Sao Paulo, Escola Paulista Med, Programa Posgrad Interdisciplinar Ciencias Saude, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental Physiology; v. 97, n. 7, p. 882-894, JUL 2012.
• Citações Web of Science: 18

Resumo

The aim of this study was to determine the effects of successive cycles of a moderately restrictive diet and refeeding with a high-fat diet on the metabolism of the adipose and hepatic tissues of obese rats. Rats were assigned to the following groups: a chow diet; a high-fat diet; a moderate caloric restriction; or a moderate caloric restriction plus refeeding. Some animals in each group were given {[}1-14C]triolein intragastrically, while others received an intraperitoneal injection of 3 mCi 3H2O. All animals were killed by decapitation. The retroperitoneal, visceral epididymal and omental white adipose tissues, brown adipose tissue, liver and blood were immediately removed. The lipid uptake from the diet, in vivo rate of lipogenesis, percentage of fat, lipid profile and leptin concentration were analysed. The high-fat diet promoted an increase in fatty liver (P= 0.05), adiposity mass (P= 0.05) and the plasma concentration of leptin (P= 0.05) and a decreased lipid uptake in white adipose tissue depots (P= 0.05) in relation to the chow diet. The moderate caloric restriction did not reverse the changes promoted by the high-fat diet but induced a small decrease in adiposity, which was reversed after refeeding, and the animals maintained a dyslipidaemic profile and high fat deposition in the liver. We can conclude that the high-fat diet and subsequent moderate caloric restriction plus refeeding increased the risks of developing visceral obesity, dyslipidaemia and non-alcoholic fatty liver disease, which suggests that this type of experimental protocol can be used to study mechanisms related to the metabolic syndrome. (AU)

• Processo FAPESP: 99/12981-7 - Estudo de propriedades funcionais do "iogurte" de soja: caracterizacao de capacidades anticarcinogenica e imunomudulatoria e identificacao de possiveis acoes sinergicas na reducao dos lipides sericos.
• Beneficiário: Elizeu Antonio Rossi
• Modalidade de apoio: Auxílio à Pesquisa - Regular