Influence of VicRK and CovR on the interactions of Streptococcus mutans with phagocytes

Autor(es)

Negrini, T. C. ^[1] ; Duque, C. ^{[1, 2]} ; Vizoto, N. L. ^[1] ; Stipp, R. N. ^[1] ; Mariano, F. S. ^[1] ; Hoefling, J. F. ^[1] ; Graner, E. ^[1] ; Mattos-Graner, R. O. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Oral Diag, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Fed Fluminense, Nova Friburgo Dent Sch, Dept Dent, Nova Friburgo, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ORAL DISEASES; v. 18, n. 5, p. 485-493, JUL 2012.
• Citações Web of Science: 6

Resumo

Objective: Streptococcus mutans are members of the oral microbiota that are implicated in dental caries and infective endocarditis. To adapt to environmental stresses encountered during host colonization, these bacteria employ two-component regulatory systems, which modulate global changes in gene expression. These include the systems VicRK and CovR. In this study, we investigate the influence of VicRK and CovR in S.mutans interactions with mononuclear and polymorphonuclear (PMN) phagocytes. Methods: Patterns of S.mutans uptake by murine macrophages were determined in strains, which differ in the production of proteins regulated by VicRK and CovR. Bacterial uptake by murine macrophages and by PMN in human blood was analyzed in vicK and covR knockout mutants obtained in strains UA159 and LT11. Results: Inactivation of covR did not affect uptake by macrophages, while vicK inactivation transiently reduced uptake only in LT11 (P < 0.05). In the two strains, inactivation of vicK and covR impaired uptake by PMN for a period of 1 h or more (P < 0.010.05). Mutant complementation with vicK or covR restored the PMN uptake phenotypes. Conclusion: This study indicates that VicRK and CovR regulate functions that influence bacterial susceptibility to phagocytosis, suggesting a novel role for these systems in the virulence of S.mutans. (AU)

• Processo FAPESP: 05/57753-4 - Analise dos padroes de imternalizacao de macrofagos de streptococcus mutans por macrofagos murinos.
• Beneficiário: Thais de Cassia Negrini
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 07/57454-2 - Efeito da inativacao de genes regulatorios e da exposicao a macrofagos murinos na expressao de glucosiltransferases b e c e de glucan-binding protein b em isolados de streptococcus mutans.
• Beneficiário: Renata de Oliveira Mattos Graner
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/02803-8 - Influência do regulador CovR na resposta de Streptococcus mutans ao contato com saliva e sangue humano
• Beneficiário: Natália Leal Vizoto
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 05/55775-0 - Análise genética da função biológica da proteína glucan-binding protein B (GbpB) de Streptococcus mutans
• Beneficiário: Cristiane Duque
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 06/55933-8 - Efeito da inativação de genes regulatórios e da exposição a macrófagos murinos na expressão de glucosiltransferases B e C e de glucan-binding protein B em isolados de Streptococcus mutans
• Beneficiário: Rafael Nobrega Stipp
• Modalidade de apoio: Bolsas no Brasil - Doutorado