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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of VicRK and CovR on the interactions of Streptococcus mutans with phagocytes

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Author(s):
Negrini, T. C. [1] ; Duque, C. [1, 2] ; Vizoto, N. L. [1] ; Stipp, R. N. [1] ; Mariano, F. S. [1] ; Hoefling, J. F. [1] ; Graner, E. [1] ; Mattos-Graner, R. O. [1]
Total Authors: 8
Affiliation:
[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Oral Diag, BR-13414903 Piracicaba, SP - Brazil
[2] Univ Fed Fluminense, Nova Friburgo Dent Sch, Dept Dent, Nova Friburgo, RJ - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ORAL DISEASES; v. 18, n. 5, p. 485-493, JUL 2012.
Web of Science Citations: 6
Abstract

Objective: Streptococcus mutans are members of the oral microbiota that are implicated in dental caries and infective endocarditis. To adapt to environmental stresses encountered during host colonization, these bacteria employ two-component regulatory systems, which modulate global changes in gene expression. These include the systems VicRK and CovR. In this study, we investigate the influence of VicRK and CovR in S.mutans interactions with mononuclear and polymorphonuclear (PMN) phagocytes. Methods: Patterns of S.mutans uptake by murine macrophages were determined in strains, which differ in the production of proteins regulated by VicRK and CovR. Bacterial uptake by murine macrophages and by PMN in human blood was analyzed in vicK and covR knockout mutants obtained in strains UA159 and LT11. Results: Inactivation of covR did not affect uptake by macrophages, while vicK inactivation transiently reduced uptake only in LT11 (P < 0.05). In the two strains, inactivation of vicK and covR impaired uptake by PMN for a period of 1 h or more (P < 0.010.05). Mutant complementation with vicK or covR restored the PMN uptake phenotypes. Conclusion: This study indicates that VicRK and CovR regulate functions that influence bacterial susceptibility to phagocytosis, suggesting a novel role for these systems in the virulence of S.mutans. (AU)

FAPESP's process: 09/02803-8 - Influence of the CovR regulator in Streptococcus mutans response to contact with saliva and blood
Grantee:Natália Leal Vizoto
Support Opportunities: Scholarships in Brazil - Master