Propidium iodide for making heterochromatin more evident in the C-banding technique

Autor(es)

Lui, R. L. ^[1] ; Blanco, D. R. ^[1] ; Moreira-Filho, O. ^[1] ; Margarido, V. P. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Genet & Evolut, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Estadual Oeste Parana, Ctr Biol & Hlth Sci, Ctr Biol & Hlth Sci, BR-5819110 Cascavel, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOTECHNIC & HISTOCHEMISTRY; v. 87, n. 7, p. 433-438, OCT 2012.
• Citações Web of Science: 31

Resumo

The detection of regions of heterochromatin has been the subject of intense investigation. We investigated an adaptation of the commonly used technique by replacing the nonfluorescent dye, Giemsa, by a fluorescent one, propidium iodide. This adaptation produces greater contrast of the heterochromatic bands in metaphase chromosomes and can be especially valuable when the organisms studied possess heterochromatin that is pale and difficult to visualize. We discuss the interactions of these two dyes with DNA and the excitation of the fluorescent dye when irradiated with ultraviolet light. (AU)

• Processo FAPESP: 09/15780-6 - Estudos evolutivos em Auchenipteridae (Siluriformes): citogenética, DNA mitocondrial e DNA satélite.
• Beneficiário: Roberto Laridondo Lui
• Modalidade de apoio: Bolsas no Brasil - Doutorado