Propidium iodide for making heterochromatin more evident in the C-banding technique

Lui, R. L.; Blanco, D. R.; Moreira-Filho, O.; Margarido, V. P.

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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Propidium iodide for making heterochromatin more evident in the C-banding technique

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Author(s):	Lui, R. L. ^[1] ; Blanco, D. R. ^[1] ; Moreira-Filho, O. ^[1] ; Margarido, V. P. ^[2] Total Authors: 4
Affiliation:	^[1] Univ Fed Sao Carlos, Dept Genet & Evolut, BR-13565905 Sao Carlos, SP - Brazil ^[2] Univ Estadual Oeste Parana, Ctr Biol & Hlth Sci, Ctr Biol & Hlth Sci, BR-5819110 Cascavel, PR - Brazil Total Affiliations: 2
Document type:	Journal article
Source:	BIOTECHNIC & HISTOCHEMISTRY; v. 87, n. 7, p. 433-438, OCT 2012.
Web of Science Citations:	31
Abstract
The detection of regions of heterochromatin has been the subject of intense investigation. We investigated an adaptation of the commonly used technique by replacing the nonfluorescent dye, Giemsa, by a fluorescent one, propidium iodide. This adaptation produces greater contrast of the heterochromatic bands in metaphase chromosomes and can be especially valuable when the organisms studied possess heterochromatin that is pale and difficult to visualize. We discuss the interactions of these two dyes with DNA and the excitation of the fluorescent dye when irradiated with ultraviolet light. (AU)

FAPESP's process:	09/15780-6 - Evolutive studies in Auchenipteridae (Siluriforms): cytogenetics, mitochondrial and satellite DNA
Grantee:	Roberto Laridondo Lui
Support Opportunities:	Scholarships in Brazil - Doctorate

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