Inflammatory and bone regulators expression in murine macrophages under exposure of commercial and experimental mineral trioxide aggregate

Autor(es)

Obando Pereda, G. ^{[1, 2]} ; Cornejo Fudinaga, A. C. ^[3] ; Salas Beltran, H. ^[3] ; Peroni, L. A. ^[1] ; Stach-Machado, D. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Anat Cell Biol & Physiol & Biophys, BR-13083970 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Inst Biol, BR-13083970 Sao Paulo - Brazil

^[3] St Mary Univ, Sch Dent, Arequipa - Peru

• Tipo de documento: Artigo Científico
• Fonte: AUSTRALIAN DENTAL JOURNAL; v. 57, n. 3, p. 284-291, SEP 2012.
• Citações Web of Science: 4

Resumo

Background: Mineral trioxide aggregate (MTA) has been used in a variety of surgical and non-surgical endodontic applications. The aim of this study was to evaluate the gene expression and protein production of TNF-alpha, IL-1 beta and IL-6, as well as the gene expression of RANKL and OPG using both commercial and experimental MTA in macrophage cell cultures. Methods: Peritoneal macrophage cell culture was performed. Viability, gene expression of cytokines, RANKL and OPG, and protein levels in experimental- and commercial-grey MTA co-cultured with peritoneal macrophages was determined by tryptan blue, real time PCR and ELISA. Results: The expression of TNF-alpha for both commercial and experimental MTA was higher, while the expression of IL-1 beta and IL-6 was similar when compared to the negative control. At protein expression level, no differences were observed between the negative control and cements. RANKL did not show a significant improvement in gene expression when compared with the negative control, but OPG expression in cement samples was higher when compared to the negative control. Conclusions: This study suggests that commercial and experimental MTA promotes anti-inflammatory processes, as well as bone healing capacity. (AU)

• Processo FAPESP: 07/58796-4 - Importancia e funcao dos receptores tlr-2 e r no reconhecimento da zirconia e titanio.
• Beneficiário: Gustavo Alberto Obando Pereda
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/56731-2 - Função e envolvimento dos receptores TLR2 e TLR4 no reconhecimento das partículas de titânio e zircônia
• Beneficiário: Dagmar Ruth Stach - Machado
• Modalidade de apoio: Auxílio à Pesquisa - Regular