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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Inflammatory and bone regulators expression in murine macrophages under exposure of commercial and experimental mineral trioxide aggregate

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Autor(es):
Obando Pereda, G. [1, 2] ; Cornejo Fudinaga, A. C. [3] ; Salas Beltran, H. [3] ; Peroni, L. A. [1] ; Stach-Machado, D. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Dept Anat Cell Biol & Physiol & Biophys, BR-13083970 Sao Paulo - Brazil
[2] Univ Estadual Campinas, Inst Biol, BR-13083970 Sao Paulo - Brazil
[3] St Mary Univ, Sch Dent, Arequipa - Peru
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: AUSTRALIAN DENTAL JOURNAL; v. 57, n. 3, p. 284-291, SEP 2012.
Citações Web of Science: 4
Resumo

Background: Mineral trioxide aggregate (MTA) has been used in a variety of surgical and non-surgical endodontic applications. The aim of this study was to evaluate the gene expression and protein production of TNF-alpha, IL-1 beta and IL-6, as well as the gene expression of RANKL and OPG using both commercial and experimental MTA in macrophage cell cultures. Methods: Peritoneal macrophage cell culture was performed. Viability, gene expression of cytokines, RANKL and OPG, and protein levels in experimental- and commercial-grey MTA co-cultured with peritoneal macrophages was determined by tryptan blue, real time PCR and ELISA. Results: The expression of TNF-alpha for both commercial and experimental MTA was higher, while the expression of IL-1 beta and IL-6 was similar when compared to the negative control. At protein expression level, no differences were observed between the negative control and cements. RANKL did not show a significant improvement in gene expression when compared with the negative control, but OPG expression in cement samples was higher when compared to the negative control. Conclusions: This study suggests that commercial and experimental MTA promotes anti-inflammatory processes, as well as bone healing capacity. (AU)

Processo FAPESP: 07/58796-4 - Importância e função dos receptores TLR-2 e r no reconhecimento da zircônia e titânio
Beneficiário:Gustavo Alberto Obando Pereda
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 07/56731-2 - Função e envolvimento dos receptores TLR2 e TLR4 no reconhecimento das partículas de titânio e zircônia
Beneficiário:Dagmar Ruth Stach - Machado
Linha de fomento: Auxílio à Pesquisa - Regular