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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Inflammatory and bone regulators expression in murine macrophages under exposure of commercial and experimental mineral trioxide aggregate

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Author(s):
Obando Pereda, G. [1, 2] ; Cornejo Fudinaga, A. C. [3] ; Salas Beltran, H. [3] ; Peroni, L. A. [1] ; Stach-Machado, D. [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, Dept Anat Cell Biol & Physiol & Biophys, BR-13083970 Sao Paulo - Brazil
[2] Univ Estadual Campinas, Inst Biol, BR-13083970 Sao Paulo - Brazil
[3] St Mary Univ, Sch Dent, Arequipa - Peru
Total Affiliations: 3
Document type: Journal article
Source: AUSTRALIAN DENTAL JOURNAL; v. 57, n. 3, p. 284-291, SEP 2012.
Web of Science Citations: 4
Abstract

Background: Mineral trioxide aggregate (MTA) has been used in a variety of surgical and non-surgical endodontic applications. The aim of this study was to evaluate the gene expression and protein production of TNF-alpha, IL-1 beta and IL-6, as well as the gene expression of RANKL and OPG using both commercial and experimental MTA in macrophage cell cultures. Methods: Peritoneal macrophage cell culture was performed. Viability, gene expression of cytokines, RANKL and OPG, and protein levels in experimental- and commercial-grey MTA co-cultured with peritoneal macrophages was determined by tryptan blue, real time PCR and ELISA. Results: The expression of TNF-alpha for both commercial and experimental MTA was higher, while the expression of IL-1 beta and IL-6 was similar when compared to the negative control. At protein expression level, no differences were observed between the negative control and cements. RANKL did not show a significant improvement in gene expression when compared with the negative control, but OPG expression in cement samples was higher when compared to the negative control. Conclusions: This study suggests that commercial and experimental MTA promotes anti-inflammatory processes, as well as bone healing capacity. (AU)