Study of the role of microRNAs on biological effects of TGF-beta1 on human dental ...
| Full text | |
| Author(s): |
Obando Pereda, G.
[1, 2]
;
Cornejo Fudinaga, A. C.
[3]
;
Salas Beltran, H.
[3]
;
Peroni, L. A.
[1]
;
Stach-Machado, D.
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Estadual Campinas, Dept Anat Cell Biol & Physiol & Biophys, BR-13083970 Sao Paulo - Brazil
[2] Univ Estadual Campinas, Inst Biol, BR-13083970 Sao Paulo - Brazil
[3] St Mary Univ, Sch Dent, Arequipa - Peru
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | AUSTRALIAN DENTAL JOURNAL; v. 57, n. 3, p. 284-291, SEP 2012. |
| Web of Science Citations: | 4 |
| Abstract | |
Background: Mineral trioxide aggregate (MTA) has been used in a variety of surgical and non-surgical endodontic applications. The aim of this study was to evaluate the gene expression and protein production of TNF-alpha, IL-1 beta and IL-6, as well as the gene expression of RANKL and OPG using both commercial and experimental MTA in macrophage cell cultures. Methods: Peritoneal macrophage cell culture was performed. Viability, gene expression of cytokines, RANKL and OPG, and protein levels in experimental- and commercial-grey MTA co-cultured with peritoneal macrophages was determined by tryptan blue, real time PCR and ELISA. Results: The expression of TNF-alpha for both commercial and experimental MTA was higher, while the expression of IL-1 beta and IL-6 was similar when compared to the negative control. At protein expression level, no differences were observed between the negative control and cements. RANKL did not show a significant improvement in gene expression when compared with the negative control, but OPG expression in cement samples was higher when compared to the negative control. Conclusions: This study suggests that commercial and experimental MTA promotes anti-inflammatory processes, as well as bone healing capacity. (AU) | |