Renin-angiotensin system may trigger kidney damage in NOD mice

Autor(es)

Colucci, Juliana Almada ^[1] ; Arita, Danielle Yuri ^[1] ; Cunha, Tatiana Sousa ^[1] ; Di Marco, Giovana Seno ^[2] ; Vio, Carlos P. ^[3] ; Pacheco-Silva, Alvaro ^[1] ; Casarini, Dulce Elena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Div Nephrol, Dept Med, Sao Paulo - Brazil

^[2] Univ Clin Muenster, Dept Internal Med D, Munster - Germany

^[3] Pontificia Univ Catolica Chile, Dept Physiol, Ctr Aging & Regenerat CARE, Santiago - Chile

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF THE RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM; v. 12, n. 1, p. 15-22, MAR 2011.
• Citações Web of Science: 6

Resumo

Diabetic nephropathy is a complication of diabetes and one of the main causes of end-stage renal disease. A possible causal link between renin-angiotensin aldosterone system (RAAS) and diabetes is widely recognized but the mechanisms by which the RAAS may lead to this complication remains unclear. The aim of this study was to evaluate angiotensin-I converting enzyme (ACE) activity and expression in numerous tissues, especially kidney, of non-obese diabetic mouse. Kidney, lung, pancreas, heart, liver and adrenal tissues from diabetic and control female NOD mice were homogenized for measurement of ACE activity, SDS-PAGE and Western blotting for ACE and ACE2, immunohistochemistry for ACE and angiotensins I, II and 1-7 and bradykinin quantification. ACE activity was higher in kidney, lung and adrenal tissue of diabetic mice compared with control mice. In pancreas, activity was decreased in the diabetic group. Western blotting analysis indicated that both groups presented ACE isoforms with molecular weights of 142 and 69 kDa and a decrease in ACE2 protein expression. Angiotensin concentrations were not altered within groups, although bradykinin levels were higher in diabetic mice. The immunohistochemical study in kidney showed an increase in tubular ACE expression. Our results show that the RAAS is affected by diabetes and the elevated ACE/ACE2 ratio may contribute to renal damage. (AU)

• Processo FAPESP: 04/15875-3 - Efeito de Diabetes Melito tipo 1 sobre a atividade do sistema renina-angiotensina em células mesangiais de camundongo NOD
• Beneficiário: Juliana Almada Colucci Barros
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 02/13290-2 - Isoforma (90 kDa) da enzima conversora de Angiotensina I, potencial marcador genético de hipertensão: processamento, caracterização molecular e funcional, e segregação genética
• Beneficiário: Dulce Elena Casarini
• Modalidade de apoio: Auxílio à Pesquisa - Temático