Effects of glutamine on the nuclear factor-kappaB signaling pathway of murine peritoneal macrophages

Autor(es)

Rogero, Marcelo Macedo ^[1] ; Borelli, Primavera ^[2] ; Fock, Ricardo Ambrosio ^[2] ; Borges, Maria Carolina ^[1] ; Ramirez Vinolo, Marco Aurelio ^[3] ; Curi, Rui ^[3] ; Nakajima, Karina ^[2] ; Crisma, Amanda Rabello ^[2] ; Ramos, Aline Domingas ^[2] ; Tirapegui, Julio ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Nutr, Sch Publ Hlth, BR-01246904 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Clin & Toxicol Anal, Fac Pharmaceut Sci, BR-05508900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Food & Expt Nutr, Fac Pharmaceut Sci, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Amino Acids; v. 39, n. 2, p. 435-441, JUL 2010.
• Citações Web of Science: 10

Resumo

The aim of this study was to evaluate the effect of glutamine on the expression of proteins involved in the nuclear factor-kappaB (NF-kappa B) signaling pathway of murine peritoneal macrophages. Since glutamine is essential for the normal functioning of macrophages, it was hypothesized that in vitro glutamine supplementation would increase NF-kappa B activation. Peritoneal macrophages were pretreated with glutamine (0, 0.6, 2 and 10 mM) before incubation with lipopolysaccharide (LPS), and the effects of glutamine on the production of tumor necrosis factor-alpha and on the expression and activity of proteins involved in the NF-kappa B signaling pathway were studied by an enzyme linked immuno-sorbent assay, Western blotting, and an electrophoretic mobility shift assay. Glutamine treatment (2 and 10 mM) increased the activation of NF-kappa B in LPS-stimulated peritoneal macrophages (P < 0.05). In non-stimulated cells, glutamine treatment (2 and 10 mM) significantly reduced I kappa B-alpha protein expression (P < 0.05). Glutamine modulates NF-kappa B signaling pathway by reducing the level of I kappa B-alpha, leading to an increase in NF-kappa B within the nucleus in peritoneal macrophages. (AU)

• Processo FAPESP: 07/53448-8 - Modulação da glutamina sobre a via de sinalização do fator nuclear kappa B (NF-kB) em macrófagos peritoniais de camundongos
• Beneficiário: Julio Orlando Tirapegui Toledo
• Modalidade de apoio: Auxílio à Pesquisa - Regular