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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of glutamine on the nuclear factor-kappaB signaling pathway of murine peritoneal macrophages

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Author(s):
Rogero, Marcelo Macedo [1] ; Borelli, Primavera [2] ; Fock, Ricardo Ambrosio [2] ; Borges, Maria Carolina [1] ; Ramirez Vinolo, Marco Aurelio [3] ; Curi, Rui [3] ; Nakajima, Karina [2] ; Crisma, Amanda Rabello [2] ; Ramos, Aline Domingas [2] ; Tirapegui, Julio [4]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Dept Nutr, Sch Publ Hlth, BR-01246904 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Clin & Toxicol Anal, Fac Pharmaceut Sci, BR-05508900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Food & Expt Nutr, Fac Pharmaceut Sci, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Amino Acids; v. 39, n. 2, p. 435-441, JUL 2010.
Web of Science Citations: 10
Abstract

The aim of this study was to evaluate the effect of glutamine on the expression of proteins involved in the nuclear factor-kappaB (NF-kappa B) signaling pathway of murine peritoneal macrophages. Since glutamine is essential for the normal functioning of macrophages, it was hypothesized that in vitro glutamine supplementation would increase NF-kappa B activation. Peritoneal macrophages were pretreated with glutamine (0, 0.6, 2 and 10 mM) before incubation with lipopolysaccharide (LPS), and the effects of glutamine on the production of tumor necrosis factor-alpha and on the expression and activity of proteins involved in the NF-kappa B signaling pathway were studied by an enzyme linked immuno-sorbent assay, Western blotting, and an electrophoretic mobility shift assay. Glutamine treatment (2 and 10 mM) increased the activation of NF-kappa B in LPS-stimulated peritoneal macrophages (P < 0.05). In non-stimulated cells, glutamine treatment (2 and 10 mM) significantly reduced I kappa B-alpha protein expression (P < 0.05). Glutamine modulates NF-kappa B signaling pathway by reducing the level of I kappa B-alpha, leading to an increase in NF-kappa B within the nucleus in peritoneal macrophages. (AU)