The effect of yerba mate (Ilex paraguariensis) aqueous extract ingestion on nuclear factor kappa B signaling pathway in peritoneal macrophages of rats fed a high-fat diet

• Grant number: 09/02371-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: September 01, 2009
• End date: August 31, 2011
• Field of knowledge: Health Sciences - Nutrition
• Principal Investigator: Marcelo Macedo Rogero
• Grantee: Maria Carolina Borges
• Host Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Obesity-induced chronic inflammation is associated with macrophages migration to adipose tissue, which produces proinflammatory cytokines, such as tumor necrosis factor (TNF)-alpha, interleukin (IL)- 1 e IL-6, contributing to type 2 diabetes mellitus and metabolic syndrome pathogenesis. In macrophages, the modulation of gene expression of many cytokines involved in inflammatory processes is controlled by a transcription factor named nuclear factor-kappa B (NF-kappa B). Yerba mate (Ilex paraguariensis) contains bioactive compounds, such as cafeic acid, kaempferol and quercetin, which reduce in vitro NF-kappaB activation in macrophages. This project aims to investigate the effect of Yerba Maté aqueous extract ingestion on NF-kappaB signaling pathway in peritoneal macrophages of rats fed a high-fat diet. Wistar male rats will be fed a control diet (n= 30) or a high-fat diet (n= 30) during 12 weeks. After this period, 10 animals from each group will be sacrificed, while the remaining animals will be distributed to two groups: one of which will receive, by gavage, Yerba mate aqueous extract (1 g/kg body weight) for four weeks. Then, the peritoneal macrophages will be collected, stimulated with lipopolysaccharides (LPS) and used to evaluate TNF-alpha, IL-1beta e IL-6 mRNAs expression; NF-kappaB activation; IKK-beta and IKB-alpha (total and phosphorylated) expression and in vitro synthesis of TNF-alpha, IL-1beta e IL-6. Body composition of animals' carcasses will be analyzed and the blood will be used for the determination of glucose, total cholesterol, HDL-cholesterol, LDL-cholesterol, VLDL-cholesterol, triacilglicerols, insulin, leptin, adiponectin, TNF-alpha, IL-6, monocyte chemotactic protein (MCP)-1, plasminogen activator inhibitor (PAI)-1, alanin aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BORGES, MARIA CAROLINA; RAMIREZ VINOLO, MARCO AURELIO; NAKAJIMA, KARINA; DE CASTRO, INAR ALVESDE; MARKOWICZ BASTOS, DEBORAH HELENA; BORELLI, PRIMAVERA; FOCK, RICARDO AMBROSIO; TIRAPEGUI, JULIO; CURI, RUI; ROGERO, MARCELO MACEDO. The effect of mate tea (Ilex paraguariensis) on metabolic and inflammatory parameters in high-fat diet-fed Wistar rats. INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, v. 64, n. 5, p. 561-569, AUG 2013. (09/02371-0)

BORGES, MARIA C.; VINOLO, MARCO A. R.; CRISMA, AMANDA R.; FOCK, RICARDO A.; BORELLI, PRIMAVERA; TIRAPEGUI, JULIO; CURI, RUI; ROGERO, MARCELO M.. High-fat diet blunts activation of the nuclear factor-kappa B signaling pathway in lipopolysaccharide-stimulated peritoneal macrophages of Wistar rats. NUTRITION, v. 29, n. 2, p. 443-449, FEB 2013. (09/02371-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BORGES, Maria Carolina. Effect of high-fat diet and yerba mate aqueous extract (Ilex paraguariensis) on body composition and on inflammatory response of peritonial macrophages in Wistar rats. 2011. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Saúde Pública (FSP/CIR) São Paulo.