Changes in bone mass, biomechanical properties, and microarchitecture of calcium- and iron-deficient rats fed diets supplemented with inulin-type fructans

Autor(es)

Lobo, Alexandre R. ^[1] ; Cocato, Maria Lucia ^[1] ; Jorgetti, Vanda ^[2] ; de Sa, Lilian R. M. ^[3] ; Nakano, Eduardo Y. ^[4] ; Colli, Celia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Nephrol, Sch Med, BR-05508900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Pathol, Sch Vet Med, BR-05508900 Sao Paulo - Brazil

^[4] Univ Brasilia, Dept Stat, BR-70910900 Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Nutrition Research; v. 29, n. 12, p. 873-881, DEC 2009.
• Citações Web of Science: 13

Resumo

Feeding mineral-deficient diets enhances absorptive efficiency as an attempt of the body to compensate for the lack of an essential nutrient. Under certain circumstances, it does not succeed, and nutritional deficiency is produced Our hypothesis was that mulin-type fructans (ITF), which arc known to affect mineral absorption, could increase Ca and Fe bioavailability in Ca- and Fe-deficient rats. Male Wistar rats (n = 48, 4 weeks old) were assigned to I of 8 groups derived from 2 x 2 x 2 factorial design with 2 levels of added Fe (0 and 35 mg/kg), Ca (0 and 5 g/kg), and ITF (0 and 100 g/kg) for 33 days. The Fe status (hemoglobin, serum Fe, total Fe-binding capacity, transferrin saturation, liver minerals) was evaluated. Tibia minerals (Ca, Mg, and Zn), bone strength, and histomorphometry were determined In nondeficient rats, ITF supplementation did not affect Fe status or organ minerals, with the exception of tibia Mg Moreover, ITF improved bone resilience and led to a reduction in eroded surface per body surface and number of osteoclasts per area In Ca-deficient rats, ITF increased liver (Fe and Zn) and tibia (Zn) mineral levels but impaired tibia Mg, yield load, and resilience. In conclusion, ITF worsened the tibia Mg levels and elastic properties when supplemented in Ca-deficient diets In contrast, although bone Ca was not affected in nondeficient rats under the present experimental conditions, bone quality improved, as demonstrated by a moderate reduction in femur osteoclast resorption and significant increases in tibia Mg content and elasticity. (C) 2009 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 06/01735-0 - Consequências fisiológicas de diferentes níveis de ingestão de ferro e cálcio e sua interação com fitatos e frutanos: estudo em ratos
• Beneficiário: Célia Colli
• Modalidade de apoio: Auxílio à Pesquisa - Regular