Pkd1 Haploinsufficiency Increases Renal Damage and Induces Microcyst Formation following Ischemia/Reperfusion

Autor(es)

Bastos, Ana P. ^[1] ; Piontek, Klaus ^[2] ; Silva, Ana M. ^[3] ; Martini, Dino ^[4] ; Menezes, Luis F. ^[2] ; Fonseca, Jonathan M. ^[1] ; Fonseca, Ivone I. ^[1] ; Germino, Gregory G. ^[2] ; Onuchic, Luiz F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Div Nephrol, Dept Med, Sao Paulo - Brazil

^[2] Johns Hopkins Univ, Sch Med, Dept Med, Div Nephrol, Baltimore, MD 21205 - USA

^[3] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo - Brazil

^[4] Beneficencia Portuguesa Hosp, Div Pathol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY; v. 20, n. 11, p. 2389-2402, NOV 2009.
• Citações Web of Science: 51

Resumo

Mutations in PKD1 cause the majority of cases of autosomal dominant polycystic kidney disease (ADPKD). Because polycystin 1 modulates cell proliferation, cell differentiation, and apoptosis, its lower biologic activity observed in ADPKD might influence the degree of injury after renal ischemia/reperfusion. We induced renal ischemia/reperfusion in 10- to 12-wk-old male noncystic Pkd1(+/-) and wild-type mice. Compared with wild-type mice, heterozygous mice had higher fractional excretions of sodium and potassium and higher serum creatinine after 48 h. In addition, in heterozygous mice, also cortical damage, rates of apoptosis, and inflammatory infiltration into the interstitium at time points out to 14 d after injury all increased, as well as cell proliferation at 48 h and 7 d. The mRNA and protein expression of p21 was lower in heterozygous mice than wild-type mice at 48 h. After 6 wk, we observed dilated tubules, microcysts, and increased renal fibrosis in heterozygotes. The early mortality of heterozygotes was significantly higher than that of wild-type mice when we extended the duration of ischemia from 32 to 35 min. In conclusion, ischemia/reperfusion induces a more severe injury in kidneys of Pkd1-haploin-sufficient mice, a process that apparently depends on a relative deficiency of p2l activity, tubular dilation, and microcyst formation. These data suggest the possibility that humans with ADPKD from PKD1 mutations may be at greater risk for damage from renal ischemia/reperfusion injury. (AU)

• Processo FAPESP: 06/52037-1 - Efeitos da expressão diferencial quantitativa do gene PKD1 sobre a lesão induzida por isquemia/reperfusão e a capacidade de regeneração renal
• Beneficiário: Luiz Fernando Onuchic
• Modalidade de apoio: Auxílio à Pesquisa - Regular