Phylogenetic analysis of PRP8 intein in Paracoccidioides brasiliensis species

Autor(es)

Theodoro, Raquel C. ^[1] ; Bagagli, Eduardo ^[1] ; Oliveira, Claudio ^[2]

Afiliação do(s) autor(es)

^[1] UNESP, Inst Biociencias, Depto Microbiol & Immunol, BR-18618000 Botucatu, SP - Brazil

^[2] UNESP, Inst Biociencias, Depto Morfol, BR-18618000 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Fungal Genetics and Biology; v. 45, n. 9, p. 1284-1291, SEP 2008.
• Citações Web of Science: 33

Resumo

A recent species status investigation of the pathogenic fungus Paracoccidioides brasiliensis suggested the existence of three cryptic species. In the present study the sequences of the PRP8 intein from A brasiliensis isolates belonging to the three described genetic groups and two unidentified isolates were determined and analyzed in order to check their functionality and usefulness for species identification. All the isolates presented a full-length intein, although the Endonuclease domain seems to be inactive due to substitutions in the second essential aspartic acid residue. Phylogenetic analysis by Maximum-Parsimony, Maximum Likelihood, and Bayesian Analysis clearly separated the isolates from the three species and revealed a significant difference between the Pb01 isolate and the remaining ones. The Pb01 isolate does not belong to any of the groups previously described since it presented a high divergence level compared to the three different genetic groups, corroborating some previous studies that suggested this isolate represents a new species of Paracoccidioides. (C) 2008 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 06/03597-4 - Ecologia e evolução do Paracoccidioides brasiliensis: avaliando a ocorrência de espécies crípticas e detecção molecular em animais silvestres em área hiper endêmica de paracoccidioidomicose
• Beneficiário: Eduardo Bagagli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/01306-5 - Espécies crípticas em Paracoccidioides brasiliensis: avaliação micológica e molecular de isolados de área hiperendêmica
• Beneficiário: Raquel Cordeiro Theodoro
• Modalidade de apoio: Bolsas no Brasil - Doutorado