Discrimination of selected species of pathogenic bacteria using near-infrared Raman spectroscopy and principal components analysis

Autor(es)

de Siqueira e Oliveira, Fernanda SantAna ^[1] ; Giana, Hector Enrique ^[2] ; Silveira, Jr., Landulfo ^[3]

Afiliação do(s) autor(es)

^[1] Univ Paulista UNIP, Inst Ciencias & Saude, BR-12240420 Sao Jose Dos Campos, SP - Brazil

^[2] Lab Oswaldo Cruz, BR-12243003 Sao Jose Dos Campos, SP - Brazil

^[3] Univ Camilo Castelo Branco UNICASTELO, Inst Biomed Engn, BR-12247004 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biomedical Optics; v. 17, n. 10 OCT 2012.
• Citações Web of Science: 20

Resumo

A method, based on Raman spectroscopy, for identification of different microorganisms involved in bacterial urinary tract infections has been proposed. Spectra were collected from different bacterial colonies (Gram-negative: Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa and Enterobacter cloacae, and Gram-positive: Staphylococcus aureus and Enterococcus spp.), grown on culture medium (agar), using a Raman spectrometer with a fiber Raman probe (830 nm). Colonies were scraped from the agar surface and placed on an aluminum foil for Raman measurements. After preprocessing, spectra were submitted to a principal component analysis and Mahalanobis distance (PCA/MD) discrimination algorithm. We found that the mean Raman spectra of different bacterial species show similar bands, and S. aureus was well characterized by strong bands related to carotenoids. PCA/MD could discriminate Gram-positive bacteria with sensitivity and specificity of 100% and Gram-negative bacteria with sensitivity ranging from 58 to 88% and specificity ranging from 87% to 99%. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). {[}DOI: 10.1117/1.JBO.17.10.107004] (AU)

• Processo FAPESP: 09/01788-5 - Espectroscopia Raman dispersiva utilizando fibras ópticas "Raman probe" aplicada ao diagnóstico de neoplasias na pele e próstata
• Beneficiário: Landulfo Silveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular