Cellular components of the human medial amygdaloid nucleus

Autor(es)

Dall'Oglio, Aline ^[1] ; Xavier, Leder L. ^[2] ; Hilbig, Arlete ^[3] ; Ferme, Denise ^[4] ; Moreira, Jorge E. ^[5] ; Achaval, Matilde ^{[1, 6]} ; Rasia-Filho, Alberto A. ^{[4, 1]}

Afiliação do(s) autor(es)

^[1] Univ Fed Rio Grande do Sul, Neurosci Grad Program, BR-90170050 Porto Alegre, RS - Brazil

^[2] Pontificia Univ Catolica Rio Grande do Sul, Fac Biosci, Lab Cell & Tissue Biol, BR-90619900 Porto Alegre, RS - Brazil

^[3] Fed Univ Hlth Sci Porto Alegre, Dept Clin Med Neurol, BR-90170050 Porto Alegre, RS - Brazil

^[4] Fed Univ Hlth Sci Porto Alegre, Dept Basic Sci Physiol, BR-90170050 Porto Alegre, RS - Brazil

^[5] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Neurosci & Behav, Dept Cell Mol Biol & Biopathogens, BR-14049900 Sao Paulo, SP - Brazil

^[6] Univ Fed Rio Grande do Sul, Dept Basic Sci Histol, BR-90170050 Porto Alegre, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF COMPARATIVE NEUROLOGY; v. 521, n. 3, p. 589-611, FEB 15 2013.
• Citações Web of Science: 9

Resumo

The medial nucleus (Me) is a superficial component of the amygdaloid complex. Here we assessed the density and morphology of the neurons and glial cells, the glial fibrillary acidic protein (GFAP) immunoreactivity, and the ultrastructure of the synaptic sites in the human Me. The optical fractionator method was applied. The Me presented an estimated mean neuronal density of 1.53 x 105 neurons/mm3 (greater in the left hemisphere), more glia (72% of all cells) than neurons, and a nonneuronal:neuronal ratio of 2.7. Golgi-impregnated neurons had round or ovoid, fusiform, angular, and polygonal cell bodies (1030 mu m in diameter). The length of the dendrites varied, and pleomorphic spines were found in sparsely spiny or densely spiny cells (1.55.2 spines/dendritic mu m). The axons in the Me neuropil were fine or coarsely beaded, and fibers showed simple or notably complex collateral terminations. The protoplasmic astrocytes were either isolated or formed small clusters and showed GFAP-immunoreactive cell bodies and multiple branches. Furthermore, we identified both asymmetrical (with various small, clear, round, electron-lucent vesicles and, occasionally, large, dense-core vesicles) and symmetrical (with small, flattened vesicles) axodendritic contacts, also including multisynaptic spines. The astrocytes surround and may compose tripartite or tetrapartite synapses, the latter including the extracellular matrix between the pre- and the postsynaptic elements. Interestingly, the terminal axons exhibited a glomerular-like structure with various asymmetrical contacts. These new morphological data on the cellular population and synaptic complexity of the human Me can contribute to our knowledge of its role in health and pathological conditions. J. Comp. Neurol. 521:589611, 2013. (c) 2012 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 09/01571-6 - Amígdala medial de ratas ao longo do ciclo estral: morfologia, expressão de mRNAs, proteínas sinápticas e lateralidade
• Beneficiário: Jorge Eduardo Moreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 03/03953-7 - Crio-preservação da ultraestrutura celular mantendo a qualidade antigênica: imunolocalização de proteínas desnaturáveis pela fixação química
• Beneficiário: Jorge Eduardo Moreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/10753-0 - Neurogênese e plasticidade sináptica no hipocampo de ratos submetidos à separação maternal e ao ambiente enriquecido
• Beneficiário: Jorge Eduardo Moreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular