Role of TLR-2 and Fungal Surface Antigens on Innate Immune Response Against Sporothrix schenckii

Autor(es)

Negrini, Thais de C. ^[1] ; Ferreira, Lucas S. ^[1] ; Alegranci, Pamela ^[1] ; Arthur, Rodrigo A. ^{[2, 3]} ; Sundfeld, Pedro P. ^[1] ; Maia, Danielle C. G. ^[1] ; Spolidorio, Luis C. ^[4] ; Carlos, Iracilda Z. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Araraquara Sch Pharmaceut Sci, Dept Clin Anal, BR-14801902 Araraquara, SP - Brazil

^[2] Univ Fed Rio Grande do Sul, Sch Dent, Dept Prevent & Community Dent, BR-90046900 Porto Alegre, RS - Brazil

^[3] Indiana Univ, Sch Dent, Dept Prevent & Community Dent, Indianapolis, IN - USA

^[4] Sao Paulo State Univ, Araraquara Sch Dent, Dept Physiol & Pathol, BR-14801902 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: IMMUNOLOGICAL INVESTIGATIONS; v. 42, n. 1, p. 36-48, 2013.
• Citações Web of Science: 27

Resumo

Sporotrichosis is an infection caused by the dimorphic fungus Sporothrix schenckii. Toll-like receptors (TLRs) play an important role in immunity, since they bind to pathogen surface antigens and initiate the immune response. However, little is known about the role of TLR-2 and fungal surface antigens in the recognition of S. schenckii and in the subsequent immune response. This study aimed to evaluate the involvement of TLR-2 and fungal surface soluble (SolAg) and lipidic (LipAg) antigens in phagocytosis of S. schenckii and production of immune mediators by macrophages obtained from WT and TLR-2(-/-) animals. The results showed that TLR-2(-/-) animals had had statistical lower percentage of macrophages with internalized yeasts compared to WT. SolAg and LipAg impaired phagocytosis and immunological mediator production for both WT and TLR-2(-/-). The absence of TLR-2 led to lower production of the cytokines TNF-alpha, IL-1 beta, IL-12 and IL-10 compared to WT animals. These results suggest a new insight in relation to how the immune system, through TLR-2, recognizes and induces the production of mediators in response to the fungus S. schenckii. (AU)

• Processo FAPESP: 09/11999-3 - Influência dos receptores TLR-2 e TLR-4 na defesa do hospedeiro contra o fungo Sporothrix schenckii
• Beneficiário: Iracilda Zeppone Carlos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/07529-1 - Influência do receptor TLR-2 na defesa do hospedeiro contra o fungo Sporothrix schenckii
• Beneficiário: Thais de Cassia Negrini
• Modalidade de apoio: Bolsas no Brasil - Doutorado