| Texto completo | |
| Autor(es): |
Araujo Gardinassi, Luiz Gustavo
[1]
;
Marques Simas, Paulo Vitor
[2]
;
Gomes, Deriane Elias
[3]
;
do Bonfim, Caroline Measso
[2]
;
Nogueira, Felipe Cavassan
[2]
;
Garcia, Gustavo Rocha
[1]
;
Aparecida Carareto, Claudia Marcia
[2]
;
Rahal, Paula
[2]
;
de Souza, Fatima Pereira
[3]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Med Ribeirao Preto, SP Dept Bioquim & Imunol, BR-14049900 Monte Alegre do Sul - Brazil
[2] Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas Sao Jos, SP Dept Biol, BR-15054000 Jardim Nazareth - Brazil
[3] Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas Sao Jos, SP Dept Fis, BR-15054000 Jardim Nazareth - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Viruses-Basel; v. 4, n. 11, p. 2432-2447, NOV 2012. |
| Citações Web of Science: | 3 |
| Resumo | |
HRSV is one of the most important pathogens causing acute respiratory tract diseases as bronchiolitis and pneumonia among infants. HRSV was isolated from two distinct communities, a public day care center and a public hospital in Sao Jose do Rio Preto - SP, Brazil. We obtained partial sequences from G gene that were used on phylogenetic and selection pressure analysis. HRSV accounted for 29% of respiratory infections in hospitalized children and 7.7% in day care center children. On phylogenetic analysis of 60 HRSV strains, 48 (80%) clustered within or adjacent to the GA1 genotype; GA5, NA1, NA2, BA-IV and SAB1 were also observed. SJRP GA1 strains presented variations among deduced amino acids composition and lost the potential O-glycosilation site at amino acid position 295, nevertheless this resulted in an insertion of two potential O-glycosilation sites at positions 296 and 297. Furthermore, a potential O-glycosilation site insertion, at position 293, was only observed for hospital strains. Using SLAC and MEME methods, only amino acid 274 was identified to be under positive selection. This is the first report on HRSV circulation and genotypes classification derived from a day care center community in Brazil. (AU) | |
| Processo FAPESP: | 10/50444-4 - Clonagem, expressão, purificação e caracterização biofísica da proteína G do vírus sincicial respiratório humano (hRSV) e estudo das interações com potenciais inibidores |
| Beneficiário: | Fátima Pereira de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |