Evaluation of histological changes after tracheal occlusion at different gestational ages in a fetal rat model

Autor(es)

Gallindo, Rodrigo Melo ^[1] ; Lanhellas Goncalves, Frances Lilian ^[1] ; de Resende Barreto, Carolina Teixeira ^[2] ; Santos Schmidt, Augusto Frederico ^[3] ; Violin Dias Pereira, Luis Antonio ^[4] ; Sbragia, Lourenco ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Surg & Anat, Div Pediat Surg, Ribeirao Preto, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Sch Med Sci, Dept Obstet & Gynecol, Campinas, SP - Brazil

^[3] Cincinnati Childrens Hosp Med Ctr, Dept Pediat, Cincinnati, OH - USA

^[4] Univ Estadual Campinas, UNICAMP, Dept Histol & Embryol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Clinics; v. 68, n. 1, p. 59-63, 2013.
• Citações Web of Science: 2

Resumo

OBJECTIVES: To evaluate the histological changes of tracheal cartilage and epithelium caused by tracheal occlusion at different gestational ages in a fetal rat model. METHODS: Rat fetuses were divided into two groups: a) External control, composed of non-operated rats, and b) Interventional group, composed of rats operated upon on gestational day 18.5 (term = 22 days), divided into triads: 1) Tracheal occlusion, 2) Internal control and 3) Sham (manipulated but not operated). Morphological data for body weight, total lung weight and total lung weight/body weight ratio were collected and measured on gestational days 19.5, 20.5 and 21.5. Tracheal samples were histologically processed, and epithelial, chondral and total tracheal thicknesses were measured on each gestational day. RESULTS: The tracheal occlusion group exhibited an increase in total lung weight/body weight ratio (p<0.001). Histologically, this group had a thicker epithelial thickness (p<0.05) and thinner chondral (p<0.05) and total tracheal thicknesses (p<0.001). These differences were more prominent on gestational days 20.5 and 21.5. CONCLUSION: Tracheal occlusion changed tracheal morphology, increased epithelial thickness and considerably decreased total tracheal thickness. These changes in the tracheal wall could explain the development of tracheomegaly, recently reported in some human fetuses subjected to tracheal occlusion. (AU)

• Processo FAPESP: 11/12587-0 - Avaliação in vivo do efeito do corticóide e da ventilação na via do óxido nítrico sobre a expressão do VEGF e seus receptores pulmonares após a oclusão traqueal fetal na hérnia diafragmática congênita em ratos
• Beneficiário: Frances Lilian Lanhellas Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/00794-1 - Avaliação da via do óxido nítrico in vivo após a oclusão traqueal fetal sobre a expressão de receptores de VEGF pulmonar na hérnia diafragmática congênita em ratos induzida pelo Nitrofen
• Beneficiário: Lourenço Sbragia Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/52772-9 - Efeito do acido retinoico sobre a expressao de receptores de vegf pulmonares em modelos animais de hernia diafragmatica congenita induzida por nitrofen.
• Beneficiário: Augusto Frederico Santos Schmidt
• Modalidade de apoio: Bolsas no Brasil - Mestrado