Corticosteroid effect upon intestinal and hepatic interleukin profile in a gastroschisis rat model

Autor(es)

Lanhellas Goncalves, Frances Lilian ^[1] ; Bittencourt, Daniel Guimaraes ^[2] ; Velloso, Licio Augusto ^[3] ; Schmidt, Augusto Frederico ^[4] ; Gallindo, Rodrigo Melo ^[1] ; Sbragia, Lourenco ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo FMRP USP, Sch Med Ribeirao Preto, Dept Surg & Anat, Div Pediat Surg, Ribeirao Preto, SP - Brazil

^[2] Hosp Municipal Dr Mario Gatti, Campinas, SP - Brazil

^[3] State Univ Campinas UNICAMP, Sch Med Sci, Dept Internal Med, Campinas, SP - Brazil

^[4] Cincinnati Childrens Hosp Med Center, Dept Pediat, Cincinnati, OH - USA

• Tipo de documento: Artigo Científico
• Fonte: Acta Cirurgica Brasileira; v. 28, n. 1, p. 8-12, 2013.
• Citações Web of Science: 1

Resumo

PURPOSE: To evaluate the effect of corticosteroids on intestinal and liver interleukin profile in an experimental model of gastroschisis in fetal rats. METHODS: Sprague-Dawley rats at 19.5 days of gestation had its fetuses operated for the creation of gastroschisis. Two groups of fetuses were studied with and without maternal administration of dexamethasone. Each group was composed of fetuses who underwent gastroschisis (G), control fetuses without manipulation (C) and sham fetuses (S). A dosage of the following interleukins was carried out in fetal intestinal and liver tissues: IL-1, IL-6, IL-10, tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma). The differences between the groups and subgroups were tested by ANOVA with Tukey post-test, with significant values of p<0.05. RESULTS: Dexamethasone led to an increase in intestinal and liver IL-6 (p<0.05) and a decrease in intestinal TNF-alpha (p<0.001) in fetuses with gastroschisis. CONCLUSION: Corticosteroids had an effect on the intestinal interleukin profile and a small effect on the liver interleukin profile due to immunological immaturity of the fetus, and also of fetuses with gastroschisis. The steroid action may not be exclusively anti-inflammatory, but also pro-inflammatory, varying with time of pregnancy. (AU)

• Processo FAPESP: 11/12587-0 - Avaliação in vivo do efeito do corticóide e da ventilação na via do óxido nítrico sobre a expressão do VEGF e seus receptores pulmonares após a oclusão traqueal fetal na hérnia diafragmática congênita em ratos
• Beneficiário: Frances Lilian Lanhellas Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/00794-1 - Avaliação da via do óxido nítrico in vivo após a oclusão traqueal fetal sobre a expressão de receptores de VEGF pulmonar na hérnia diafragmática congênita em ratos induzida pelo Nitrofen
• Beneficiário: Lourenço Sbragia Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 02/02563-8 - Avaliação morfológica, histológica e bioquímica de fígado de fetos de ratas Spreague-Dowley, submetidos à gastrosquise experimental intra-útero, em diferentes idades gestacionais
• Beneficiário: Lourenço Sbragia Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular