Corticosteroid effect upon intestinal and hepatic interleukin profile in a gastroschisis rat model

Author(s)

Lanhellas Goncalves, Frances Lilian ^[1] ; Bittencourt, Daniel Guimaraes ^[2] ; Velloso, Licio Augusto ^[3] ; Schmidt, Augusto Frederico ^[4] ; Gallindo, Rodrigo Melo ^[1] ; Sbragia, Lourenco ^[1]

Affiliation

^[1] Univ Sao Paulo FMRP USP, Sch Med Ribeirao Preto, Dept Surg & Anat, Div Pediat Surg, Ribeirao Preto, SP - Brazil

^[2] Hosp Municipal Dr Mario Gatti, Campinas, SP - Brazil

^[3] State Univ Campinas UNICAMP, Sch Med Sci, Dept Internal Med, Campinas, SP - Brazil

^[4] Cincinnati Childrens Hosp Med Center, Dept Pediat, Cincinnati, OH - USA

• Document type: Journal article
• Source: Acta Cirurgica Brasileira; v. 28, n. 1, p. 8-12, 2013.
• Web of Science Citations: 1

Abstract

PURPOSE: To evaluate the effect of corticosteroids on intestinal and liver interleukin profile in an experimental model of gastroschisis in fetal rats. METHODS: Sprague-Dawley rats at 19.5 days of gestation had its fetuses operated for the creation of gastroschisis. Two groups of fetuses were studied with and without maternal administration of dexamethasone. Each group was composed of fetuses who underwent gastroschisis (G), control fetuses without manipulation (C) and sham fetuses (S). A dosage of the following interleukins was carried out in fetal intestinal and liver tissues: IL-1, IL-6, IL-10, tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma). The differences between the groups and subgroups were tested by ANOVA with Tukey post-test, with significant values of p<0.05. RESULTS: Dexamethasone led to an increase in intestinal and liver IL-6 (p<0.05) and a decrease in intestinal TNF-alpha (p<0.001) in fetuses with gastroschisis. CONCLUSION: Corticosteroids had an effect on the intestinal interleukin profile and a small effect on the liver interleukin profile due to immunological immaturity of the fetus, and also of fetuses with gastroschisis. The steroid action may not be exclusively anti-inflammatory, but also pro-inflammatory, varying with time of pregnancy. (AU)

• FAPESP's process: 11/12587-0 - Evaluation in vivo of the effect of corticosteroids and ventilation in oxide nitric pathway on the expression of VEGF and its pulmonary receptors after fetal tracheal occlusion in the congenital diaphragmatic hernia in rats
• Grantee: Frances Lilian Lanhellas Gonçalves
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/00794-1 - Assessment of nitric oxide (NO) in vivo after fetal tracheal occlusion on the expression of VEGF receptors in pulmonary congenital diaphragmatic hernia induced by Nitrofen
• Grantee: Lourenço Sbragia Neto
• Support Opportunities: Regular Research Grants