Effect of nitrofen in the final stages of development of the diaphragm muscle in rats

Autor(es)

Lanhellas Goncalves, Frances Lilian ^[1] ; de Oliveira, Fabio Santana ^[2] ; Schmidt, Augusto Frederico ^[3] ; Violin Dias Pereira, Luis Antonio ^[4] ; Gallindo, Rodrigo Melo ^[1] ; Figueira, Rebeca Lopes ^[1] ; Sbragia, Lourenco ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo FMRP USP, Sch Med Ribeirao Preto, Dept Surg & Anat, Div Pediat Surg, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo FM USP, Sch Med, Sao Paulo - Brazil

^[3] Cincinnati Childrens Hosp Med Ctr, Dept Pediat, Cincinnati, OH - USA

^[4] State Univ Campinas UNICAMP, Dept Histol & Embryol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Acta Cirurgica Brasileira; v. 28, n. 1, p. 13-18, 2013.
• Citações Web of Science: 1

Resumo

PURPOSE: To evaluate the expression of myosin in muscle fibers of the diaphragm in experimental congenital diaphragmatic hernia (CDH). METHODS: Fetuses of pregnant rats were divided into four groups: External Control (EC), composed of non-manipulated rats; Nitrofen, composed of pregnant rats that received 100 mg of nitrofen (2,4-dichloro-4' nitrodiphenyl ether) diluted in olive oil on gestational day (GD) 9.5, whose fetuses developed CDH (N+) or not (N-), and Olive Oil Placebo (OO), composed of pregnant rats that received the oil on the same GD. The fetuses were collected on GD 18.5, 19.5, 20.5 and 21.5 (term = 22 days). We obtained body weight (BW) and photographed the diaphragm area (DA), hernia area (HA) and subsequent calculated the HA/DA ratio in N+ group. Samples of Diaphragm muscle were processed for histological staining with H/E and immunohistochemistry (IHQ) for myosin. RESULTS: The fetuses of N- and N+ groups had decreased BW and DA compared to EC and OO groups (p < 0.001). HA was decreased on GD 18.5 compared to 21.5 (p < 0.001) and the HA/DA ratio showed no difference. IHQ showed decreased expression of myosin in nitrofen groups. CONCLUSION: CDH induced by nitrofen model contributes to the understanding of muscularization in the formation of the diaphragm where the myosin expression is decreased. (AU)

• Processo FAPESP: 11/12587-0 - Avaliação in vivo do efeito do corticóide e da ventilação na via do óxido nítrico sobre a expressão do VEGF e seus receptores pulmonares após a oclusão traqueal fetal na hérnia diafragmática congênita em ratos
• Beneficiário: Frances Lilian Lanhellas Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/00794-1 - Avaliação da via do óxido nítrico in vivo após a oclusão traqueal fetal sobre a expressão de receptores de VEGF pulmonar na hérnia diafragmática congênita em ratos induzida pelo Nitrofen
• Beneficiário: Lourenço Sbragia Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular