Effect of nitrofen in the final stages of development of the diaphragm muscle in rats

Author(s)

Lanhellas Goncalves, Frances Lilian ^[1] ; de Oliveira, Fabio Santana ^[2] ; Schmidt, Augusto Frederico ^[3] ; Violin Dias Pereira, Luis Antonio ^[4] ; Gallindo, Rodrigo Melo ^[1] ; Figueira, Rebeca Lopes ^[1] ; Sbragia, Lourenco ^[1]

Affiliation

^[1] Univ Sao Paulo FMRP USP, Sch Med Ribeirao Preto, Dept Surg & Anat, Div Pediat Surg, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo FM USP, Sch Med, Sao Paulo - Brazil

^[3] Cincinnati Childrens Hosp Med Ctr, Dept Pediat, Cincinnati, OH - USA

^[4] State Univ Campinas UNICAMP, Dept Histol & Embryol, Campinas, SP - Brazil

• Document type: Journal article
• Source: Acta Cirurgica Brasileira; v. 28, n. 1, p. 13-18, 2013.
• Web of Science Citations: 1

Abstract

PURPOSE: To evaluate the expression of myosin in muscle fibers of the diaphragm in experimental congenital diaphragmatic hernia (CDH). METHODS: Fetuses of pregnant rats were divided into four groups: External Control (EC), composed of non-manipulated rats; Nitrofen, composed of pregnant rats that received 100 mg of nitrofen (2,4-dichloro-4' nitrodiphenyl ether) diluted in olive oil on gestational day (GD) 9.5, whose fetuses developed CDH (N+) or not (N-), and Olive Oil Placebo (OO), composed of pregnant rats that received the oil on the same GD. The fetuses were collected on GD 18.5, 19.5, 20.5 and 21.5 (term = 22 days). We obtained body weight (BW) and photographed the diaphragm area (DA), hernia area (HA) and subsequent calculated the HA/DA ratio in N+ group. Samples of Diaphragm muscle were processed for histological staining with H/E and immunohistochemistry (IHQ) for myosin. RESULTS: The fetuses of N- and N+ groups had decreased BW and DA compared to EC and OO groups (p < 0.001). HA was decreased on GD 18.5 compared to 21.5 (p < 0.001) and the HA/DA ratio showed no difference. IHQ showed decreased expression of myosin in nitrofen groups. CONCLUSION: CDH induced by nitrofen model contributes to the understanding of muscularization in the formation of the diaphragm where the myosin expression is decreased. (AU)

• FAPESP's process: 11/00794-1 - Assessment of nitric oxide (NO) in vivo after fetal tracheal occlusion on the expression of VEGF receptors in pulmonary congenital diaphragmatic hernia induced by Nitrofen
• Grantee: Lourenço Sbragia Neto
• Support type: Regular Research Grants
• FAPESP's process: 11/12587-0 - Evaluation in vivo of the effect of corticosteroids and ventilation in oxide nitric pathway on the expression of VEGF and its pulmonary receptors after fetal tracheal occlusion in the congenital diaphragmatic hernia in rats
• Grantee: Frances Lilian Lanhellas Gonçalves
• Support type: Scholarships in Brazil - Post-Doctorate