E-cadherin in canine mast cell tumors: Decreased expression and altered subcellular localization in Grade 3 tumors

Autor(es)

Mackowiak, I. I. ^[1] ; Gentile, L. B. ^[1] ; Chaible, L. M. ^[1] ; Nagamine, M. K. ^[1] ; Guerra, J. M. ^[1] ; Mota, E. F. F. ^[1] ; Matera, J. M. ^[2] ; Mennecier, G. ^[1] ; Sanches, D. S. ^[1] ; Dagli, M. L. Z. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Lab Expt & Comparat Oncol, Dept Pathol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Surg, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VETERINARY JOURNAL; v. 194, n. 3, p. 405-411, DEC 2012.
• Citações Web of Science: 1

Resumo

Mast cell tumors (MCTs) are the most frequent round cell tumors in dogs and comprise approximately 21% of all canine cutaneous tumors. MCTs are highly invasive and metastatic corresponding to the histological grade. E-cadherin is an adhesion molecule expressed in epithelial cells and although it is an epithelial cellular marker, studies have shown expression of E-cadherin in canine round cell tumors. To better characterize the expression pattern of E-cadherin in several different histological grades of MCTs in dogs, the expression and localization of the adhesion molecule was investigated using immunohistochemistry. For this purpose, 18 cutaneous MCTs were classified into three histological grades, 1, 2 or 3. Clinical history and follow-up data were available for all of the dogs. Cytoplasmic and nuclear expressions of E-cadherin in all three types of tumors were verified by immunostaining using two different antibodies. There was decreased E-cadherin expression in the more aggressive MCTs (Grade 3), suggesting an association between E-cadherin and tumor aggressiveness. Additionally, the loss of E-cadherin expression in either the cytoplasm or nucleus in more aggressive and undifferentiated tumor types confirmed the importance of cellular adhesion in tumor behavior. (C) 2012 Published by Elsevier Ltd. (AU)

• Processo FAPESP: 10/08523-4 - Avaliação da expressão do c-kit, PTEN e correlação entre as conexinas 32, 43, E-caderina e a proteínas citoplasmáticas associadas a caderinas em mastocitomas da espécie canina
• Beneficiário: Maria Lucia Zaidan Dagli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/12827-1 - Avaliação da expressão e correlação entre as conexinas 32, 43, E-caderina e proteínas citoplasmáticas associadas a E-caderinas em mastocitomas da espécie canina.
• Beneficiário: Ivone Izabel Mackowiak da Fonseca
• Modalidade de apoio: Bolsas no Brasil - Mestrado