Altered sleep regulation in a mouse model of SCN1A-derived genetic epilepsy with febrile seizures plus (GEFS+)

Autor(es)

Papale, Ligia A. ^{[1, 2]} ; Makinson, Christopher D. ^[2] ; Ehlen, J. Christopher ^[3] ; Tufik, Sergio ^[1] ; Decker, Michael J. ^[4] ; Paul, Ketema N. ^[3] ; Escayg, Andrew ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Psychobiol, Sao Paulo - Brazil

^[2] Emory Univ, Dept Human Genet, Atlanta, GA 30322 - USA

^[3] Morehouse Sch Med, Dept Neurobiol, Atlanta, GA 30310 - USA

^[4] Georgia State Univ, Sch Nursing & Hlth Profess, Atlanta, GA 30303 - USA

• Tipo de documento: Artigo Científico
• Fonte: Epilepsia; v. 54, n. 4, p. 625-634, APR 2013.
• Citações Web of Science: 23

Resumo

Purpose Mutations in the voltage-gated sodium channel (VGSC) gene SCN1A are responsible for a number of epilepsy disorders, including genetic epilepsy with febrile seizures plus (GEFS+) and Dravet syndrome. In addition to seizures, patients with SCN1A mutations often experience sleep abnormalities, suggesting that SCN1A may also play a role in the neuronal pathways involved in the regulation of sleep. However, to date, a role for SCN1A in the regulation of sleep architecture has not been directly examined. To fill this gap, we tested the hypothesis that SCN1A contributes to the regulation of sleep architecture, and by extension, that SCN1A dysfunction contributes to the sleep abnormalities observed in patients with SCN1A mutations. Methods Using immunohistochemistry we first examined the expression of mouse Scn1a in regions of the mouse brain that are known to be involved in seizure generation and sleep regulation. Next, we performed detailed analysis of sleep and wake electroencephalography (EEG) patterns during 48 continuous hours of baseline recordings in a knock-in mouse line that expresses the human SCN1A GEFS+ mutation R1648H (RH mutants). We also characterized the sleepwake pattern following 6h of sleep deprivation. Key Findings Immunohistochemistry revealed broad expression of Scn1a in the neocortex, hippocampus, hypothalamus, thalamic reticular nuclei, dorsal raphe nuclei, pedunculopontine, and laterodorsal tegmental nuclei. Co-localization between Scn1a immunoreactivity and critical cell types within these regions was also observed. EEG analysis under baseline conditions revealed increased wakefulness and reduced nonrapid eye movement (NREM) and rapid eye movement (REM) sleep amounts during the dark phase in the RH mutants, suggesting a sleep deficit. Nevertheless, the mutants exhibited levels of NREM and REM sleep that were generally similar to wild-type littermates during the recovery period following 6 h of sleep deprivation. Significance These results establish a direct role for SCN1A in the regulation of sleep and suggest that patients with SCN1A mutations may experience chronic alterations in sleep, potentially leading to negative outcomes over time. In addition, the expression of Scn1a in specific cell types/brain regions that are known to play critical roles in seizure generation and sleep now provides a mechanistic basis for the clinical features (seizures and sleep abnormalities) associated with human SCN1A mutations. (AU)

• Processo FAPESP: 07/50534-0 - Caracterização do padrão de sono em modelos animais de epilepsia
• Beneficiário: Ligia Assumpção Papale
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 98/14303-3 - Center for Sleep Studies
• Beneficiário: Sergio Tufik
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs