Curcumin-mediated photodynamic inactivation of Candida albicans in a murine model of oral candidiasis

Autor(es)

Dovigo, Livia Nordi ^[1] ; Carmello, Juliana Cabrini ^[2] ; de Souza Costa, Carlos Alberto ^[3] ; Vergani, Carlos Eduardo ^[2] ; Brunetti, Iguatemy Lourenco ^[4] ; Bagnato, Vanderlei Salvador ^[5] ; Pavarina, Ana Claudia ^[2]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Dept Social Dent, Sao Paulo - Brazil

^[2] UNESP Univ Estadual Paulista, Dept Dent Mat & Prosthodont, Sao Paulo - Brazil

^[3] UNESP Univ Estadual Paulista, Dept Physiol & Pathol, Araraquara Dent Sch, Sao Paulo - Brazil

^[4] UNESP Univ Estadual Paulista, Sch Pharmaceut Sci, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Phys Inst Sao Carlos, Biophoton Lab, Grp Opt, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Medical Mycology; v. 51, n. 3, p. 243-251, APR 2013.
• Citações Web of Science: 51

Resumo

In vitro investigations of curcumin-mediated photodynamic therapy (PDT) are encouraging, but there is a lack of reliable in vivo evidence of its efficacy. This study describes the photoinactivation of Candida albicans in a murine model of oral candidiasis, using curcumin as a photosensitizer. Forty immunosuppressed mice were orally inoculated with C. albicans and after five days, they received topical curcumin (20, 40 and 80 mu M) and illumination with LED light. The use of curcumin or light alone were also investigated. Positive control animals did not receive any treatment and negative control animals were not inoculated with C. albicans. The number of surviving yeast cells was determined and analyzed by ANOVA and Tukey's post-hoc test (alpha = 0.05). Histological evaluation of the presence of yeast and inflammatory reaction was also conducted. All exposures to curcumin with LED light caused a significant reduction in C. albicans viability after PDT, but the use of 80 mu M curcumin associated with light was able to induce the highest log 10 reduction in colony counts (4 logs). It was concluded that curcumin-mediated PDT proved to be effective for in vivo inactivation of C. albicans without harming the host tissue of mice. (AU)

• Processo FAPESP: 08/00601-6 - Estudo da efetividade da Terapia Fotodinâmica Antimicrobiana na inativação de espécies de Candida e do dano celular causado nas células fúngicas. Avaliação in vitro e in vivo de dois fotossensibilizadores.
• Beneficiário: Lívia Nordi Dovigo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/03994-9 - Estudo da efetividade da terapia fotodinâmica antimicrobiana na inativação de espécies de Candida e do dano celular causado nas células fúngicas: avaliação in vitro e in vivo de dois fotossensibilizadores
• Beneficiário: Ana Cláudia Pavarina
• Modalidade de apoio: Auxílio à Pesquisa - Regular