A novel strategy of mesenchymal stem cells delivery in the uterus of mares with endometrosis

Autor(es)

Mambelli, L. I. ^{[1, 2]} ; Winter, G. H. Z. ^[3] ; Kerkis, A. ^[1] ; Malschitzky, E. ^[4] ; Mattos, R. C. ^[3] ; Kerkis, I. ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Genet Lab, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Programa Posgrad Anat Anim Domest & Silvestres, Sao Paulo - Brazil

^[3] Univ Fed Rio Grande do Sul, Reprolab, Fac Med Vet, Porto Alegre, RS - Brazil

^[4] Univ Luterana Brasil, Curso Med Vet, Canoas, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 79, n. 5, p. 744-750, MAR 15 2013.
• Citações Web of Science: 17

Resumo

Mesenchymal stem cells (MSCs), because of their immunomodulation and trophic activities, in addition to their capacity to regenerate damaged tissues, have potential for treatment of many diseases. The success of stem cell therapies depends, in part, on the method of cell delivery, which should provide wide cell distribution and homing in to injured sites. The objective of the present study was to developing a novel strategy for delivery of MSCs into the uterus of mares with endometrosis (degenerative alteration of uterine glands and surrounding stroma). Endometrosis was confirmed in all mares (N = 6) used in this study. To trace multipotent equine adipose tissue-derived MSCs (eAT-MSCs) in endometrial tissue, before transplantation, cells were stained with a fluorescent dye. During a synchronized estrus, the eAT-MSCs (2 x 10(7) diluted in 20 mL of sodium chloride 0.9%) were inoculated into uterus using a simple technique, similar to artificial insemination (AI) in mares. At 7 and 21 days after transplantation, homing of fluorescently labeled eAT-MSCs was observed by confocal microscopy of uterine biopsies collected from the uterine body and in both uterine horns, including glandular and periglandular spaces, in three of four treated mares. Herein, we propose a new method of MSCs delivery in uterus of mares with endometrosis, which was minimally invasive and technically simple. (c) 2013 Published by Elsevier Inc. (AU)

• Processo FAPESP: 09/06764-7 - Células Progenitoras de Tecido Adiposo de Equinos. Estudo do seu Potencial para o Tratamento da Endometrose.
• Beneficiário: Lisley Inata Mambelli
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 07/50212-3 - Células tronco isoladas de tecido adiposo de equinos: diferenciação osteogênica, condrogênica e adipogênica in vitro
• Beneficiário: Lisley Inata Mambelli
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 06/51839-7 - Utilização de células-tronco autólogas para tratamento de lesões em equinos e outros animais
• Beneficiário: Alexandre Kerkis
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE