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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A novel strategy of mesenchymal stem cells delivery in the uterus of mares with endometrosis

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Author(s):
Mambelli, L. I. [1, 2] ; Winter, G. H. Z. [3] ; Kerkis, A. [1] ; Malschitzky, E. [4] ; Mattos, R. C. [3] ; Kerkis, I. [1]
Total Authors: 6
Affiliation:
[1] Inst Butantan, Genet Lab, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Programa Posgrad Anat Anim Domest & Silvestres, Sao Paulo - Brazil
[3] Univ Fed Rio Grande do Sul, Reprolab, Fac Med Vet, Porto Alegre, RS - Brazil
[4] Univ Luterana Brasil, Curso Med Vet, Canoas, RS - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Theriogenology; v. 79, n. 5, p. 744-750, MAR 15 2013.
Web of Science Citations: 17
Abstract

Mesenchymal stem cells (MSCs), because of their immunomodulation and trophic activities, in addition to their capacity to regenerate damaged tissues, have potential for treatment of many diseases. The success of stem cell therapies depends, in part, on the method of cell delivery, which should provide wide cell distribution and homing in to injured sites. The objective of the present study was to developing a novel strategy for delivery of MSCs into the uterus of mares with endometrosis (degenerative alteration of uterine glands and surrounding stroma). Endometrosis was confirmed in all mares (N = 6) used in this study. To trace multipotent equine adipose tissue-derived MSCs (eAT-MSCs) in endometrial tissue, before transplantation, cells were stained with a fluorescent dye. During a synchronized estrus, the eAT-MSCs (2 x 10(7) diluted in 20 mL of sodium chloride 0.9%) were inoculated into uterus using a simple technique, similar to artificial insemination (AI) in mares. At 7 and 21 days after transplantation, homing of fluorescently labeled eAT-MSCs was observed by confocal microscopy of uterine biopsies collected from the uterine body and in both uterine horns, including glandular and periglandular spaces, in three of four treated mares. Herein, we propose a new method of MSCs delivery in uterus of mares with endometrosis, which was minimally invasive and technically simple. (c) 2013 Published by Elsevier Inc. (AU)

FAPESP's process: 09/06764-7 - Equine Adipose Tissue-Derived Progenitor Cells. Study of their Potential for the Treatment of Endometrosis.
Grantee:Lisley Inata Mambelli
Support Opportunities: Scholarships in Brazil - Master